PXD072478-1

PXD072478 is an original dataset announced via ProteomeXchange.

Title	TRIM28 is an E3 ligase of IRP2 suppressing ischemia/reperfusion-induced myocardial ferroptosis
Description	Myocardial ischemia/reperfusion (I/R) injury is a common and severe clinical complication in patients with ischemic heart disease (IHD) after reperfusion therapy. Effective therapeutic strategies for myocardial I/R injury remain limited. Ferroptosis is a form of regulated cell death characterized by iron-dependent lipid peroxidation. However, the mechanisms underlying ferroptosis in myocardial I/R injury are not fully understood. METHODS: Transcriptomic data from patients with heart failure and cardiomyocytes undergoing ferroptosis were analyzed. Based on the screening results, tripartite motif-containing 28 (TRIM28) expression was evaluated in ferroptotic cardiomyocytes. Cardiac I/R injury models in mice and hypoxia/reoxygenation (H/R) injury models in neonatal rat ventricular myocytes were established. To explore the function of TRIM28, we employed adeno-associated virus serotype 9 to achieve cardiomyocyte-specific overexpression, and generated tamoxifen-inducible cardiomyocyte-specific TRIM28 knockout mice. RNA sequencing, co-immunoprecipitation coupled with mass spectrometry, and ubiquitinome profiling were applied to elucidate the underlying mechanisms. Human heart samples from patients with IHD were used to evaluate the expression of TRIM28 and its related signaling molecules. The Connectivity Map database was used to screen potential activators of TRIM28. RESULTS: We found that TRIM28 expression was downregulated in ferroptosis inducer-treated and H/R-injured cardiomyocytes, as well as in I/R-injured mouse hearts. Cardiomyocyte-specific overexpression of TRIM28 protected heart against I/R-induced ferroptosis, whereas its deficiency exacerbated myocardial I/R-induced ferroptotic injury. Mechanistically, TRIM28 functioned as an E3 ubiquitin ligase that directly bound to IRP2 and promoted K48-linked ubiquitination at the K877 site, leading to the downregulation of IRP2 and TFR1, suppression of intracellular iron uptake, and consequent attenuation of cardiomyocyte ferroptosis. Furthermore, p55γ interacted with and upregulated TRIM28, thereby mitigating I/R-induced myocardial ferroptosis. Consistent with these findings, protein levels of TRIM28 and p55γ were decreased in heart samples from patients with IHD, while IRP2 and TFR1 were increased. Lastly, we demonstrated that perhexiline inhibited I/R-induced myocardial ferroptosis by upregulating p55γ and TRIM28. CONCLUSIONS: Our study identify TRIM28 as an essential E3 ubiquitin ligase of IRP2 and delineate TRIM28-mediated inhibition of myocardial ferroptosis by targeting IRP2-TFR1 signaling protects the heart against I/R injury, indicating that targeting TRIM28 represents a promising therapeutic strategy for suppressing cardiomyocyte ferroptosis and I/R injury.
HostingRepository	iProX
AnnounceDate	2025-12-28
AnnouncementXML	Submission_2026-06-01_19:18:19.724.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kun Zhu
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-28 19:43:38	ID requested
⏵ 1	2026-06-01 19:18:20	announced

Dataset with its publication pending

submitter keyword: ferroptosis, ischemia/reperfusion, IRP2, perhexiline, TRIM28

Chunmei Cao
contact affiliation	Laboratory of Cardiovascular Science, Beijing Clinical Research Institute, Beijing Friendship Hospital, Capital Medical University
contact email	caochunmei@pku.edu.cn
lab head
Kun Zhu
contact affiliation	Laboratory of Cardiovascular Science, Beijing Clinical Research Institute, Beijing Friendship Hospital, Capital Medical University
contact email	zhukun817@163.com
dataset submitter

iProX dataset URI