PXD072415-1

PXD072415 is an original dataset announced via ProteomeXchange.

Title	3D Mechanical Stimulation Modulates Endothelial Exosomes to Promote Fibroblast Activation
Description	Background: Mechanical forces play a crucial role in regulating cellular communication during tissue repair, yet how mechanical stimulation modulates endothelial exosome secretion and their effects on fibroblast activation remains unclear. Methods: In this study, endothelial cells were incorporated into 3D bioprinted tissue-engineered dermal constructs and cultured under static or mechanically stretched conditions. Exosomes were isolated, characterized, and applied to human dermal fibroblasts to assess their influence on proliferation, migration, and extracellular matrix formation. Data-independent acquisition proteomics was performed to analyze exosomal protein cargo and associated signaling pathways. Results: Mechanical loading increased exosome secretion by approximately 2.5-fold without altering vesicle morphology. Functionally, mechanically stimulated exosomes significantly enhanced fibroblast migration and type I collagen synthesis compared with controls. Proteomic profiling identified 4,476 proteins, of which 677 were differentially expressed. Enrichment analysis revealed activation of VEGF, HIF-1, Relaxin, and AGE–RAGE pathways, implicating roles in angiogenesis, metabolic regulation, and extracellular matrix remodeling. Conclusion: These findings demonstrate that 3D mechanical stimulation not only augments the quantity of endothelial exosomes but also reshapes their molecular cargo, thereby enhancing biomechanical communication between endothelial cells and fibroblasts. Together with prior evidence of fibroblast-derived exosomes promoting endothelial angiogenesis, this study proposes a bidirectional “mechanical stimulation–exosome–communication–tissue reconstruction” loop, providing a theoretical foundation for optimizing exosome-based strategies in skin tissue engineering.
HostingRepository	iProX
AnnounceDate	2025-12-25
AnnouncementXML	Submission_2025-12-24_22:54:06.706.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ye Qiu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-24 22:53:43	ID requested
⏵ 1	2025-12-24 22:54:07	announced

Dataset with its publication pending

submitter keyword: Mechanical stimulation, Endothelial exosomes, Fibroblast activation, Mechanotransduction

Ye Qiu
contact affiliation	The First Affiliated Hospital of Soochow University
contact email	18001545968@163.com
lab head
Ye Qiu
contact affiliation	The First Affiliated Hospital of Soochow University
contact email	18001545968@163.com
dataset submitter

iProX dataset URI