PXD072412-1
PXD072412 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | GSNOR drives age-related obesity by promoting adipose tissue whitening through de-nitrosation of Beclin-1 |
| Description | Age-related obesity is a growing public health concern since it contributes to a multitude of metabolic disorders, yet its mechanisms remain poorly defined. Herein, we report that S-nitrosoglutathione reductase (GSNOR), a pivotal enzyme for de-nitrosation, significantly increased in adipose tissues in both naturally middle-aged mice and human. Knocking out (KO) GSNOR in mice leads to weight reduction, diminished adipose mass and improved metabolism, while adipose tissue-specific overexpression of GSNOR (KI) results in obesity and metabolism decline. Further study showed that aged GSNOR KO mice had higher mitochondrial content and beige adipocytes, but adipose-specific GSNOR overexpression promoted adipose tissue whitening. Quantitative S-nitrosation proteomic analysis indicate S-nitrosation of Beclin-1 at cysteine 351 decreased significantly in aging mice and GSNOR KI mice. Beclin-1C351A mutation promoted autophagy by facilitating the interaction between Beclin-1 and ATG14, thereby accelerating the conversion of beige to white adipocytes. Notably, age-related obesity is abrogated by specific knocking down GSNOR in adipose tissue. This study reveals a new mechanism and a novel therapeutic avenue for managing age-related obesity. |
| HostingRepository | iProX |
| AnnounceDate | 2025-12-25 |
| AnnouncementXML | Submission_2025-12-24_19:43:40.290.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Shilong Li |
| SpeciesList | scientific name: Mus |
| ModificationList | S-nitrosyl-L-cysteine |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-24 19:43:25 | ID requested | |
| ⏵ 1 | 2025-12-24 19:43:40 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: GSNOR, age-related obesity, S-nitrosation/S-nitrosylation, Beclin-1, autophagy, adipose tissue whitening |
Contact List
| Chang Chen | |
|---|---|
| contact affiliation | Institute of Biophysics, Chinese Academy of Sciences |
| contact email | changchen@ibp.ac.cn |
| lab head | |
| Shilong Li | |
| contact affiliation | Institute of Biophsics, Chinese Academy of Sciences |
| contact email | lishilong221@mails.ucas.ac.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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