PXD072364-1
PXD072364 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of phosphorylation in HCoV-229E-infected immortalized human lung epithelial cells (A549) |
| Description | TMT18-labeled proteome-wide profiling of post-translational modifications (phosphorylation) in mock-infected and infected human lung epithelial cells (A549). Human lung epithelial (A549) cells were infected and mock-infected with HCoV-229E. Cells were harvested at 8, 16, and 24 hours post-infection. Extracted proteins were digested with LysC + Trypsin, then TMT18-labeled and enriched for phosphorylation. Samples were split into 6 fractions and run on the Orbitrap Exploris 480. Data was searched with MS-GF+ using PNNL's DMS Processing pipeline. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-12-24 |
| AnnouncementXML | Submission_2025-12-24_16:57:10.683.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Matthew Monroe |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Human coronavirus 229E; NCBI TaxID: 11137; |
| ModificationList | Oxidation; Nethylmaleimide; Phospho; TMTpro |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-23 12:42:46 | ID requested | |
| ⏵ 1 | 2025-12-24 16:57:11 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: HCoV-229E, coronavirus, human lung, post-translational modifications, phosphorylation, DatasetType:Proteomics |
Contact List
| Tong Zhang | |
|---|---|
| contact affiliation | Pacific Northwest National Laboratory |
| contact email | tong.zhang@pnnl.gov |
| lab head | |
| Amy Sims | |
| contact affiliation | Pacific Northwest National Laboratory |
| contact email | amy.sims@pnnl.gov |
| lab head | |
| Matthew Monroe | |
| contact affiliation | Pacific Northwest National Laboratory |
| contact email | matthew.monroe@pnnl.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100324/ |
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