PXD072359 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | BioID2 interactome of wild-type SYCP1 versus the D918–942 mutant |
| Description | This dataset comprises a tandem mass tag (TMT)–based quantitative proteomic analysis of proteins proximal to SYCP1 identified using BioID proximity labelling. Two independent BioID experiments were performed using distinct SYCP1 constructs: an N-terminal Myc-tagged SYCP1 and a C-terminal HA-tagged SYCP1. For each construct, full-length wild-type SYCP1, a SYCP1 deletion mutant lacking amino acids 918–942, and a SYCP1 knockout negative control were analysed in MCF7 cells. Biotinylated proteins were enriched by streptavidin-based affinity purification, and the resulting protein lysates were submitted to the EMBL Proteomics Core Facility for downstream processing and LC–MS/MS analysis, enabling quantitative comparison of SYCP1-associated proteins across constructs and conditions. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-30 |
| AnnouncementXML | Submission_2026-04-30_12:18:07.745.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Frank Stein |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-12-23 11:43:13 | ID requested | |
| ⏵ 1 | 2026-04-30 12:18:08 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: SYCP1 |
| BioID |
| proximity labeling |
Contact List
| Dr. Urszula McClurg |
|---|
| contact affiliation | University Of Liverpool |
| contact email | Urszula.Mcclurg@liverpool.ac.uk |
| lab head | |
| Frank Stein |
|---|
| contact affiliation | EMBL |
| contact email | frank.stein@embl.de |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD072359 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD072359
- Label: PRIDE project
- Name: BioID2 interactome of wild-type SYCP1 versus the D918–942 mutant
