PXD072329-1

PXD072329 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic analysis of redox PTMs in HCoV-229E-infected immortalized human lung epithelial cells (A549)
Description	TMT18-labeled proteome-wide profiling of post-translational modifications (redox) in mock-infected and infected human lung epithelial cells (A549). Human lung epithelial (A549) cells were infected and mock-infected with HCoV-229E. Cells were harvested at 8, 16, and 24 hours post-infection. Extracted proteins were digested with LysC + Trypsin, then TMT18-labeled and enriched for redox PTMs. Samples were split into 6 fractions and run on the Orbitrap Exploris 480. Data was searched with MS-GF+ using PNNL's DMS Processing pipeline.
HostingRepository	MassIVE
AnnounceDate	2025-12-24
AnnouncementXML	Submission_2025-12-24_16:57:05.340.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew Monroe
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Human coronavirus 229E; NCBI TaxID: 11137;
ModificationList	Oxidation; Carbamidomethyl; Nethylmaleimide; TMTpro
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-22 19:17:39	ID requested
⏵ 1	2025-12-24 16:57:05	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: HCoV-229E, coronavirus, human lung, post-translational modifications, redox, DatasetType:Proteomics

submitter keyword: HCoV-229E, coronavirus, human lung, post-translational modifications, redox, DatasetType:Proteomics

Contact List

Tong Zhang
contact affiliation	Pacific Northwest National Laboratory
contact email	tong.zhang@pnnl.gov
lab head
Amy Sims
contact affiliation	Pacific Northwest National Laboratory
contact email	amy.sims@pnnl.gov
lab head
Matthew Monroe
contact affiliation	Pacific Northwest National Laboratory
contact email	matthew.monroe@pnnl.gov
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100313/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100313/