PXD072307-1

PXD072307 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of black tiger prawn (Penaeus monodon) and white leg prawn (Litopenaeus vannamei) allergen profiles
Description	Shellfish allergy is a major global health concern, with prawns being among the most common elicitors of allergic reactions. This project describes a comparative allergenomic analysis of the most widely consumed prawn species worldwide: black tiger prawn (Penaeus monodon) and white leg prawn (Litopenaeus vannamei). The aim of the study was to comprehensively characterize and compare their allergen profiles, with a particular focus on relative allergen abundance and species-specific expression patterns. Raw protein extracts from P. monodon and L. vannamei were analyzed using label-free shotgun proteomics by LC–MS/MS. Protein identification and quantification were performed using standard database searching workflows. Allergen candidates were predicted in silico using AllerCatPro 2.0, and crustacean allergens with strong evidence were selected for downstream analysis. Relative allergen abundance was assessed using intensity-based absolute quantification (iBAQ%), while differential expression between species was evaluated using label-free quantification (LFQ) intensities. Although allergens constituted a small proportion of the identified proteome, they accounted for a substantial fraction of total protein abundance in both species. Major known prawn allergens, including myosin light chain, arginine kinase, sarcoplasmic calcium-binding protein, and tropomyosin, were detected in both species, alongside several low-abundance proteins identified as potential novel allergen candidates. Species-specific differences in allergen expression were observed at the level of allergen orthologs, isoforms, and variants, with subsets of allergens uniquely detected in each species. This dataset provides a resource for studying species-specific variation in prawn allergen composition and abundance and supports future work on shellfish development and standardisation of allergy diagnostics, therapeutic strategies, and risk assessment.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_16:13:30.314.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Janitha Iddagoda
SpeciesList	scientific name: Penaeus; NCBI TaxID: NEWT:133894;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-22 13:01:13	ID requested
⏵ 1	2026-09-06 16:13:31	announced

10.1021/acs.jproteome.5c01289;

Iddagoda J, Balu VV, Nugraha R, Chin R, Karnaneedi S, Leeming MG, Williamson NA, Lopata AL, Ruethers T, ). J Proteome Res, 25(7):3253-3262(2026) [pubmed]

submitter keyword: None

Prof. Andreas
contact affiliation	Molecular Allergy Research Laboratory, Centre for Sustainable Tropical Fisheries and Aquaculture (CSTFA), College of Science and Engineering, James Cook University, Douglas, QLD 4811, Australia Tropical Futures Institute, James Cook University, 149 Sims Drive, Singapore 387380, Singapore
contact email	andreas.lopata@jcu.edu.au
lab head
Janitha Iddagoda
contact affiliation	Tropical Futures Institute, James Cook University, Singapore
contact email	janithahansineeiddagoda.iddagodagedona@my.jcu.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD072307

PRIDE project URI

• PRIDE
  1. PXD072307
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of black tiger prawn (Penaeus monodon) and white leg prawn (Litopenaeus vannamei) allergen profiles