PXD072283-1

PXD072283 is an original dataset announced via ProteomeXchange.

Title	Lysine Pyruvylation Mediated by HAT1/p300-SIRT3 Couples Glycolytic Flux to Epigenetic Regulation
Description	Metabolite-coupled post-translation modifications (PTMs) plays a crucial role in the regulation of gene expression and protein functions. In this study, we present the identification and characterization of a novel protein modification, named lysine pyruvatylation (Kpy). We identified five pyruvatylation sites on core histones and 68 sites on non-histone proteins in mammal cells. The levels of Kpy can be increased by adding sodium pyruvate and are influenced by changes in the glycolytic pathway. Through a screening of potential enzymes, we identified SIRT3 as the “eraser” of Kpy, while HAT1 and p300 function as the “writers”. Collectively, this study uncovers a new type of PTM, pyruvatylation, which is derived from pyruvate, and reveals the key regulatory elements for the Kpy pathway, laying a foundation for studying its roles in diverse cellular processes.
HostingRepository	iProX
AnnounceDate	2025-12-21
AnnouncementXML	Submission_2026-05-17_20:48:28.665.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	He Huang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	oxidation
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-22 00:38:53	ID requested
⏵ 1	2026-05-17 20:48:29	announced

Dataset with its publication pending

submitter keyword: Pyruvatylation, post-translation modification

He Huang
contact affiliation	Chinese Academy of Sciences Shanghai Institute of Materia Medica
contact email	hhuang.nu@gmail.com
lab head
He Huang
contact affiliation	Chinese Academy of Sciences Shanghai Institute of Materia Medica
contact email	hhuang.nu@gmail.com
dataset submitter

iProX dataset URI