PXD072186-1

PXD072186 is an original dataset announced via ProteomeXchange.

Title	Comprehensive proteomics and phosphorylation analysis revealed the negative role of VaVIP1 in cold tolerance of grapevine.
Description	Grapevine, a globally important fruit crop. Low temperature limits grapevine yield, quality and geographical distribution. Protein post-translational modification is involved in cold tolerance of grapevine. In order to cultivate new varieties of grapevine cold tolerance, we conducted an integrated proteomic and phosphoproteomic analysis of Vitis amurensis, identifying widespread changes involving 4,797 proteins, 10,104 phosphorylation sites and 56 motif sequences. Cold treatment resulted in 165 differential phosphorylation proteins and 635 differential phosphorylation sites. GO enrichment showed that differential phosphorylation proteins were mainly concentrated in biological processes, cellular components and molecular functions. Functional enrichment highlighted critical roles for metabolic and signaling pathways, including phenylpropanoid biosynthesis and MAPK cascades. From this dataset, we focused on the bZIP transcription factor VaVIP1 and uncovered its negative regulatory role in cold tolerance. VaVIP1 overexpression can make the grapevine damage more severe, as evidenced by increased oxidative stress, membrane damage, and attenuated osmotic adjustment. On the molecular mechanism, VaVIP1 overexpression suppressed the cold-induced activation of key genes in the CBF-regulatory pathway. Our work not only expands the phosphoproteomic resource for plants but also delineates a novel mechanism where VaVIP1 acts as a negative regulator to cold tolerance, providing a valuable theoretical foundation for improving cold tolerance in grapevine.
HostingRepository	iProX
AnnounceDate	2025-12-18
AnnouncementXML	Submission_2026-05-06_18:20:38.723.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiuming Zhang
SpeciesList	scientific name: Vitis vinifera; NCBI TaxID: 29760;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-19 00:32:09	ID requested
⏵ 1	2026-05-06 18:20:39	announced

Dataset with its publication pending

submitter keyword: grapevine, phosphoproteomic, post-translational phosphorylation sites, VaVIP1, cold

Xiuming Zhang
contact affiliation	Ningxia University
contact email	zhangxiuming@nxu.edu.cn
lab head
Xiuming Zhang
contact affiliation	Ningxia University
contact email	zhangxiuming@nxu.edu.cn
dataset submitter

iProX dataset URI