PXD072183-1

PXD072183 is an original dataset announced via ProteomeXchange.

Title	A metabolic complex of acyltransferase enzymes involved in tomato acylsugar biosynthesis
Description	Specialized metabolites mediate diverse plant-environment interactions. Recent work has begun to enzymatically characterize entire plant specialized metabolic pathways; however, little is known about how different pathway components organize and interact within the cell. Here we use acylsugars - a class of specialized metabolites – to explore metabolic complex formation. In Solanum lycopersicum (tomato) four trichome-localized acylsugar acyltransferases (SlASAT1-4) sequentially add acyl chains to a sucrose core leading to accumulation of tri and tetraacylated sucroses. Confocal microscopy demonstrates that tomato ASATs localize to distinct subcellular compartments, including the mitochondria, cytosol, and endoplasmic reticulum. To explore pairwise protein-protein interactions in acylsugar biosynthesis, we used various techniques relying on different interaction principles, including co-immunoprecipitation, split luciferase assays, and bimolecular fluorescence complementation all demonstrating pairwise ASAT interactions. Following transient expression of SlASAT1-4 in N. benthamiana, we were able to pull down a complex consisting of SlASAT1-4, which was confirmed through proteomics. Size exclusion chromatography of the SlASAT pulldown suggests a heteromultimeric complex of around 300 kDa. This study sheds light on the metabolic coordination of acylsugar biosynthesis through formation of a metabolic complex coordinating production of chemical defenses.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-27_12:40:40.838.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Craig Schenck
SpeciesList	scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: NEWT:4097; scientific name: Solanum lycopersicum; NCBI TaxID: NEWT:4081;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-18 20:05:57	ID requested
⏵ 1	2026-04-27 12:40:41	announced

10.1093/JXB/ERAG180;

submitter keyword: Solanum lycopersicum,acylsugars,SlASAT1-4

Craig Schenck
contact affiliation	University of missouri
contact email	caschenck@missouri.edu
lab head
Craig Schenck
contact affiliation	University of Missouri
contact email	caschenck@missouri.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD072183

PRIDE project URI

• PRIDE
  1. PXD072183
     1. Label: PRIDE project
     2. Name: A metabolic complex of acyltransferase enzymes involved in tomato acylsugar biosynthesis