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PXD072133-1

PXD072133 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAtlantic herring oocyte and sperm proteomes
DescriptionAs part of a major genomic study of the evolution of Atlantic herring (Clupea harengus), deep proteomes were obtained from the oocytes and sperm of herring. Three biological replicates of each tissue were used. The proteins were extracted and fractionated using basic reversed-phase (bRP) chromatography. Twelve fractions from each replicate were analyzed using LC-MS/MS. Mass spectrometry data were acquired in data-dependent acquisition (DDA) mode using an Orbitrap Exploris 480 instrument. The raw data were processed and searched using MaxQuant. Protein identification was performed using a reference proteome database of Clupea harengus supplemented with sequences of three additional LRRC8C2 alleles. LRRC8C2 is a sperm-specific volume-regulated ion channel that is the focus of a major evolutionary study. As a result, more than 4,000 and 6,000 protein groups were identified in sperm and oocytes, respectively. The Baltic North allele of LRRC8C2 was unambiguously expressed.
HostingRepositoryMassIVE
AnnounceDate2026-04-28
AnnouncementXMLSubmission_2026-04-28_02:43:00.499.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSergey Moshkovskiy
SpeciesList scientific name: Clupea harengus; common name: Atlantic herring; NCBI TaxID: 7950;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-12-18 00:27:37ID requested
12026-04-28 02:43:01announced
Publication List
no publication
Keyword List
submitter keyword: Atlantic herring, oocyte, sperm, data dependent acquisition, label-free quantitation, LRRC8C, DatasetType:Proteomics
Contact List
Prof. Henning Urlaub
contact affiliationMax Planck Institute for Multidisciplinary Sciences
contact emailhenning.urlaub@mpinat.mpg.de
lab head
Sergey Moshkovskiy
contact emailsergey.moshkovskiy@mpinat.mpg.de
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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