⮝ Full datasets listing
PXD072133
PXD072133 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Atlantic herring oocyte and sperm proteomes |
| Description | As part of a major genomic study of the evolution of Atlantic herring (Clupea harengus), deep proteomes were obtained from the oocytes and sperm of herring. Three biological replicates of each tissue were used. The proteins were extracted and fractionated using basic reversed-phase (bRP) chromatography. Twelve fractions from each replicate were analyzed using LC-MS/MS. Mass spectrometry data were acquired in data-dependent acquisition (DDA) mode using an Orbitrap Exploris 480 instrument. The raw data were processed and searched using MaxQuant. Protein identification was performed using a reference proteome database of Clupea harengus supplemented with sequences of three additional LRRC8C2 alleles. LRRC8C2 is a sperm-specific volume-regulated ion channel that is the focus of a major evolutionary study. As a result, more than 4,000 and 6,000 protein groups were identified in sperm and oocytes, respectively. The Baltic North allele of LRRC8C2 was unambiguously expressed. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-04-28 |
| AnnouncementXML | Submission_2026-04-28_02:43:00.499.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sergey Moshkovskiy |
| SpeciesList | scientific name: Clupea harengus; common name: Atlantic herring; NCBI TaxID: 7950; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-18 00:27:37 | ID requested | |
| ⏵ 1 | 2026-04-28 02:43:01 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Atlantic herring, oocyte, sperm, data dependent acquisition, label-free quantitation, LRRC8C, DatasetType:Proteomics |
Contact List
| Prof. Henning Urlaub | |
|---|---|
| contact affiliation | Max Planck Institute for Multidisciplinary Sciences |
| contact email | henning.urlaub@mpinat.mpg.de |
| lab head | |
| Sergey Moshkovskiy | |
| contact email | sergey.moshkovskiy@mpinat.mpg.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100270/ |
