PXD072115-1

PXD072115 is an original dataset announced via ProteomeXchange.

Title	Peptidomics Mapping of Proteolysis Highlights Triple Activation of Sprouted Seeds by Germination, Homogenisation and Species Mixture.
Description	We investigated how seed proteolysis was enhanced by germination, by subsequent homogenisation (disrupting sprout compartments), and by co-incubation of homogenates from different species. Mass spectrometry of released peptides tracked proteolytic signatures from chickpea, lentil, mung and broccoli proteins, in soaked seeds, in sprouted seeds, and after sprout homogenisation followed by incubation alone or in mixture with other sprouts. The proteolytic signatures differed markedly among the four species, and in the different treatment conditions. After homogenisation, legumain-like cleavage (after asparagine) increased in lentil, and proline-rich peptides increased in broccoli. For co-incubated homogenised sprouts, each species’ homogenate significantly contributed 6 to 57% of proteolytic patterns in peptides of other species, with chickpea and broccoli homogenates notably releasing metabolic protein peptides from mung and lentil. Thus, germination, homogenisation and homogenate species mixtures can each contribute to proteolysis of seed peptides, potentially increasing digestibility and reducing allergenicity
HostingRepository	PRIDE
AnnounceDate	2026-05-05
AnnouncementXML	Submission_2026-05-05_08:41:58.235.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Indrani Bera
SpeciesList	scientific name: Pisum sativum (Garden pea); NCBI TaxID: NEWT:3888; scientific name: Vigna radiata; NCBI TaxID: NCBITaxon:157791; scientific name: Cicer arietinum (Chickpea) (Garbanzo); NCBI TaxID: NEWT:3827; scientific name: Brassica oleracea var. oleracea; NCBI TaxID: NCBITaxon:109376;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-17 13:45:46	ID requested
⏵ 1	2026-05-05 08:41:59	announced
2	2026-05-05 14:25:39	announced	2026-05-05: Updated project metadata.
3	2026-05-05 16:15:41	announced	2026-05-06: Updated project metadata.

10.1016/J.FOODCHEM.2026.149370;

10.1016/j.foodchem.2026.149370;

submitter keyword: None

Denis Shields
contact affiliation	School of Medicine, Conway Institute, University College Dublin
contact email	denis.shields@ucd.ie
lab head
Indrani Bera
contact affiliation	UCD Dublin
contact email	indrani.bera@ucd.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD072115

PRIDE project URI

• PRIDE
  1. PXD072115
     1. Label: PRIDE project
     2. Name: Peptidomics Mapping of Proteolysis Highlights Triple Activation of Sprouted Seeds by Germination, Homogenisation and Species Mixture.