⮝ Full datasets listing
PXD072101-1
PXD072101 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Methicillin treatment reveals that FtsZ phosphorylation influences the cell division of Streptococcus pneumoniae |
| Description | Protein phosphorylation plays a crucial role in regulating cell division and morphogenesis in many bacteria. In Streptococcus pneumoniae, the serine/threonine kinase StkP orchestrates cell wall assembly and cell morphogenesis by phosphorylating several proteins involved in cell division. In this study, we provide evidence that FtsZ, a conserved tubulin-like protein that coordinates pneumococcal cell elongation and constriction, is phosphorylated in vivo by StkP at six phosphothreonine residues within its C-terminal linker (CTL). Mutational analysis reveals the structural role of the CTL in pneumococcal cell division and further shows that its phosphorylation status impacts cell morphogenesis. Importantly, phosphomimetic FtsZ mutants rescue division blocks induced by sublethal concentrations of methicillin, which targets the essential septal cell wall synthase PBP2x. Additionally, we demonstrate that CTL phosphorylation alters influences FtsZ polymerization and filament bundling in vitro. It also accelerates FtsZ treadmilling dynamics in vivo. Altogether, these findings support a model in which CTL phosphorylation fine-tunes FtsZ filament dynamics to sustain cell division under Beta-lactam stress, representing a potential adaptive mechanism for antibiotic tolerance. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-05-06 |
| AnnouncementXML | Submission_2026-05-06_08:05:59.701.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | DELOLME |
| SpeciesList | scientific name: Streptococcus pneumoniae; NCBI TaxID: 1313; |
| ModificationList | Phospho |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-17 08:43:34 | ID requested | |
| ⏵ 1 | 2026-05-06 08:06:00 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: FtsZ, divisome, interactants, phosphorylation, DatasetType:Proteomics |
Contact List
| Christophe GRANGEASSE | |
|---|---|
| contact affiliation | MMSB-UMR5086-CNRS |
| contact email | christophe.grangeasse@cnrs.fr |
| lab head | |
| DELOLME | |
| contact affiliation | CNRS |
| contact email | frederic.delolme@ibcp.fr |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100256/ |
