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PXD072096-1

PXD072096 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProlonged stagnation reduces treated wastewater biostability by altering microbial community balance: insights from metaproteomics
DescriptionIn this study, we integrated metagenome sequencing and metaproteomics to obtain comprehensive taxonomic and functional profiles of microbial communities, investigating the impact of stagnation compared with continuous flow dynamics. To achieve this, we employed laboratory-scale reactors fed with membrane bioreactor (MBR) effluent. The objectives were to evaluate: (i) how stagnation affects the diversity and taxonomic composition of microbial genes and proteins; (ii) how prolonged stagnation over three, five, and seven months drives temporal changes in community and functional dynamics; (iii) which functions are enriched or depleted under stagnation; and (iv) how these taxonomic and functional shifts relate to the ecological quality and biostability of reclaimed water. By understanding not only what microbes are present but also what they are doing through metaproteomics, we can assess how microbial activities impact water quality. This approach enables evaluation of treated wastewater quality both post-treatment and during distribution and storage in irrigation systems.
HostingRepositoryPRIDE
AnnounceDate2026-09-07
AnnouncementXMLSubmission_2026-09-06_16:32:16.782.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPapita Mandal
SpeciesList scientific name: wastewater metagenome; NCBI TaxID: NEWT:527639;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-12-17 06:30:51ID requested
12026-09-06 16:32:17announced
Publication List
10.1111/1462-2920.70372;
Almulhim F, Narayanasamy S, Wang C, Mandal P, Bensaddek D, Amad M, Hong PY, Prolonged Stagnation Reduces Treated Wastewater Biostability by Altering Microbial Community: Insights From Metaproteomics. Environ Microbiol, 28(7):e70372(2026) [pubmed]
Keyword List
submitter keyword: Treated wastewater
hydraulic stagnation
biofilm microbiome
metagenomics
metaproteomics
nitrogen cycling
microbial biostability
water reuse
Contact List
Pei-Ying Hong
contact affiliationProfessor,Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
contact emailpeiying.hong@kaust.edu.sa
lab head
Papita Mandal
contact affiliationStaff Scientist
contact emailpapita.mandal@kaust.edu.sa
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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