PXD072095-1

PXD072095 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Protein interactors of 5-FU in MDA-MB-231 cells
Description	This dataset originates from a Proteome Integral Solubility Alteration (PISA) experiment designed to assess how 5-fluorouracil (5-FU) alters protein thermal stability/solubility in MDA-MB-231 cells. Cells were treated with 200 μM 5-FU or DMSO for 2 h, exposed to a temperature gradient, lysed, and soluble proteins from each condition were isolated, digested, and labeled with TMTpro reagents. Labeled peptides were fractionated and analyzed by LC-MS/MS. The raw data were processed with MaxQuant against the human UniProt reference proteome, providing proteome-wide quantitative profiles of 5-FU-induced changes in protein solubility.
HostingRepository	PRIDE
AnnounceDate	2026-02-16
AnnouncementXML	Submission_2026-02-15_17:01:24.497.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-17 06:27:18	ID requested
⏵ 1	2026-02-15 17:01:25	announced

Publication List

10.1186/s12964-025-02640-y;
Sayed AE, Gomes N, Zakrzewski M, Liudkovska V, Serwa R, Azzi A, The mTORC2 component SIN1 post-transcriptionally regulates TYMS levels and modulates P53 activity in response to 5-FU chemotherapy. Cell Commun Signal, 24(1):111(2026) [pubmed]

10.1186/s12964-025-02640-y;

Sayed AE, Gomes N, Zakrzewski M, Liudkovska V, Serwa R, Azzi A, The mTORC2 component SIN1 post-transcriptionally regulates TYMS levels and modulates P53 activity in response to 5-FU chemotherapy. Cell Commun Signal, 24(1):111(2026) [pubmed]

Keyword List

submitter keyword: 5-fluorouracil, MDA-MB-231, 5-FU, PISA

submitter keyword: 5-fluorouracil, MDA-MB-231, 5-FU, PISA

Contact List

Dr. Abdelhalim Azzi
contact affiliation	IMol Polish Academy of Sciences, Warsaw, Poland
contact email	a.azzi@imol.edu.pl
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD072095
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD072095

PRIDE project URI

Repository Record List

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