PXD072077-1

PXD072077 is an original dataset announced via ProteomeXchange.

Title	CDK9 interacts with a RanGTP-NEMP1-Importin-β complex to regulate erythroid enucleation
Description	Erythroid enucleation is the final stage of erythroid terminal differentiation and involves the separation of an orthochromatic erythroblast into two daughter cells; a pyrenocyte containing the extruded nucleus, and a reticulocyte that will become a red blood cell. Our previous work identified CDK9 as a regulator of erythroid enucleation that appears to act independently of its known role in regulating RNA polymerase II transcription, suggesting the potential for a new CDK9 role. Using a co-immunoprecipitation and mass spectrometry approach, we identified the interactome of CDK9 in differentiating erythroblasts. We show that CDK9 interacts with a RanGTP-NEMP1-Importin-β complex during erythroid terminal differentiation, and inhibition of importin-β in erythroblasts blocks erythroid enucleation. Using imaging analysis and functional assays of enucleating erythroblasts, we show that CDK9 and importin-β co-locate at a critical site of activity opposite to the nucleus before nuclear extrusion and we describe a novel finding that physically links CDK9 and importin-β activity prior to CaM/Ca2+ signalling and subsequent F-actin activity to achieve enucleation.
HostingRepository	PRIDE
AnnounceDate	2026-04-01
AnnouncementXML	Submission_2026-03-31_23:45:28.091.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rohan Lowe
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-16 22:39:24	ID requested
⏵ 1	2026-03-31 23:45:28	announced

Dataset with its publication pending

submitter keyword: Enucleation, CDK9, erythropoiesis, importin-beta

Patrick O. Humbert
contact affiliation	La Trobe Institute for Molecular Science, Department of Biochemistry & Chemistry, La Trobe University, Melbourne, VIC, 3073, Australia.
contact email	p.humbert@latrobe.edu.au
lab head
Rohan Lowe
contact affiliation	La Trobe University
contact email	r.lowe@latrobe.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD072077

PRIDE project URI

• PRIDE
  1. PXD072077
     1. Label: PRIDE project
     2. Name: CDK9 interacts with a RanGTP-NEMP1-Importin-β complex to regulate erythroid enucleation