PXD072060 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Physical, chemical, and structural properties of human gastric organoid-derived mucus |
| Description | We explored the potential of human gastric organoids as models for mucus production. Immunofluorescence staining confirmed that the organoids produced mucus containing MUC5AC and MUC6. The luminal mucus had viscoelastic properties similar to those of native human gastric mucus, as determined by particle tracking microrheology. To collect organoid-produced gastric mucus, termed bioengineered gastric mucus (BGM), organoids were cultured as monolayers at the air-liquid interface (ALI), and apically-secreted mucus was harvested and analyzed by MUC5AC ELISA, proteomics, CryoFE-SEM, and bulk rheometry. BGM contained high-molecular weight molecules also found in native gastric mucus, including MUC5AC. Proteomic analysis confirmed that BGM contained MUC5AC, MUC6, MUC1, and other stomach-specific molecules such as gastricsin, olfactomedin 4, and gastrokine. CryoFE-SEM showed that both BGM and native mucus had a porous structure and a characteristic honeycomb scaffold. Bulk rheometry confirmed that BGM exhibited shear thinning and predominantly elastic behavior, consistent with native mucus. Collectively, these findings indicate that BGM is an accessible alternative to native gastric mucus that can be produced on-demand for in vitro studies. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-30 |
| AnnouncementXML | Submission_2025-12-30_14:09:54.353.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Diane Bimczok |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: NEWT:9825; |
| ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Bruker Daltonics timsTOF series |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-12-16 15:07:15 | ID requested | |
| ⏵ 1 | 2025-12-30 14:09:55 | announced | |
Publication List
| 10.1101/2025.11.08.687257; |
| Lyon KN, Sidar B, Dudiak C, Vermulm C, Jordan G, Sebrell TA, Burcham A, Domanico L, Helm RF, Liao W, Davis B, Brown J, McCalla SG, Wilking JN, Bansil R, Bimczok D, Physical, chemical, and structural properties of human gastric organoid-derived mucus. bioRxiv, ():(2025) [pubmed] |
Keyword List
| submitter keyword: gastric mucus layer |
| organoids |
| air-liquid interface |
| gastric epithelial barrier |
| microrheology |
| organoid lumen |
Contact List
| Richard Helm |
|---|
| contact affiliation | Department of Biochemistry Virginia Tech Mass Spectrometry Incubator |
| contact email | helmrf@vt.edu |
| lab head | |
| Diane Bimczok |
|---|
| contact affiliation | Montana State University |
| contact email | diane.bimczok@montana.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD072060
- Label: PRIDE project
- Name: Physical, chemical, and structural properties of human gastric organoid-derived mucus
