PXD072038-1

PXD072038 is an original dataset announced via ProteomeXchange.

Title	Functional enrichment analysis of differentially expressed proteins
Description	First, each sample (30.00 mg) was weighed and transferred to a 2 mL centrifuge tube, followed by adding two 5 mm magnetic beads, an appropriate amount of SDS-containing protein lysis buffer, and 1× Cocktail (protease inhibitor) with a final concentration of EDTA. The samples were disrupted and lysed using an automatic grinder, then centrifuged at 25,000g at 4℃ for 15 minutes to collect the supernatant. Next, DTT was added to the supernatant to a final concentration of 10 mM, and the mixture was incubated in a 37℃ water bath for 30 minutes. Subsequently, IAM was added to a final concentration of 55 mM, and the samples were placed in a dark room for 45 minutes. Five volumes of pre-cooled acetone were then added, and the mixture was stored in a -20℃ refrigerator for 2 hours, followed by centrifugation at 25,000g at 4℃ for 15 minutes to discard the supernatant. The precipitate was air-dried, and an appropriate amount of SDS-free protein lysis buffer was added. An automatic grinder was used to promote protein dissolution, and the mixture was centrifuged again at 25,000g at 4℃ for 15 minutes. The resulting supernatant was the final protein solution, which was used for subsequent quantification and analysis.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_16:15:11.986.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	dai ruihui
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: NEWT:9031;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-16 01:53:35	ID requested
⏵ 1	2026-06-07 16:15:12	announced

10.1038/s41598-026-44135-x;

Feng Y, Li X, Liu S, Tian L, Fan X, Zhao P, Dai R, Li N, Dietary quercetagetin enhances antioxidant capacity and modulates intestinal microbiota in dexamethasone-challenged broilers. Sci Rep, 16(1):(2026) [pubmed]

submitter keyword: quantitative proteomics，Gene Ontology ，Kyoto Encyclopedia of Genes and Genomes，SCP2

Ruihui Dai
contact affiliation	Department of Science and Engineering, Hebei Agricultural University
contact email	dairuihui@yeah.net
lab head
dai ruihui
contact affiliation	Hebei Agricultural University
contact email	dairuihui@yeah.net
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD072038

PRIDE project URI

• PRIDE
  1. PXD072038
     1. Label: PRIDE project
     2. Name: Functional enrichment analysis of differentially expressed proteins