PXD072028 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Hydraplexing: Signal Amplification via Additive Fragment Ion Contributions from Multi-Tag Isobaric Labeling |
| Description | This project investigates hydraplexing, a chemical strategy that increases the detectable fragment-ion signal from labeled analytes in mass spectrometry (MS). The approach introduces several isobaric barcodes onto the same molecule, so that fragmentation yields multiple tag-derived fragments whose intensities accumulate. Using a tetra-amine scaffold derivatized with 2, 3, or 4 TMT tags, we characterized fragmentation patterns under higher-energy collisional dissociation (HCD), determined HCD energy settings that balance precursor transmission with efficient barcode release in MS2, and assessed quantitative performance across direct infusion, liquid chromatography (LC), and capillary electrophoresis (CE) workflows. We further examined performance in a complex environment by analyzing hydraplexed standards spiked into a single-cell Xenopus laevis digest. These experiments demonstrate that fragment-level signal summation can improve quantitative sensitivity in metabolomics- and proteomics-style analyses and suggest routes for extending multi-barcode architectures to broader MSⁿ applications. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-26 |
| AnnouncementXML | Submission_2026-08-26_10:05:45.688.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Peter Nemes |
| SpeciesList | scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: NEWT:8355; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-12-15 21:00:54 | ID requested | |
| ⏵ 1 | 2026-08-26 10:05:46 | announced | |
Publication List
Keyword List
| submitter keyword: isobaric tagging |
| tandem mass tag |
| reporter ions |
| complementary ions |
| nanoLC–PRM |
| capillary electrophoresis |
| single-cell proteomics |
| signal amplification |
| quantitative mass spectrometry |
Contact List
| Peter Nemes |
|---|
| contact affiliation | Department of Chemistry and Biochemistry, University of Maryland, College Park |
| contact email | nemes@umd.edu |
| lab head | |
| Peter Nemes |
|---|
| contact affiliation | University of Maryland, College Park |
| contact email | nemes@umd.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD072028
- Label: PRIDE project
- Name: Hydraplexing: Signal Amplification via Additive Fragment Ion Contributions from Multi-Tag Isobaric Labeling
