⮝ Full datasets listing
PXD072016-1
PXD072016 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Phosphoproteomics analysis of MAP4K3 KO HEK293 cells +/- amino acid treatment, Al La Spada Lab |
| Description | Quantitative LC-MS/MS was performed on 3 uL of each sample, using a nanoAcquity UPLC system (Waters Corp) coupled to a Thermo Orbitrap Fusion Lumos high resolution accurate mass tandem mass spectrometer (Thermo) via a nanoelectrospray ionization source. Briefly, the sample was first trapped on a Symmetry C18 20 mm x 180 um trapping column (5 ul/min at 99.9/0.1 v/v water/acetonitrile), after which the analytical separation was performed using a 1.8 um Acquity HSS T3 C18 75 um x 250 mm column (Waters Corp.) with a 60-min linear gradient of 3 to 30% acetonitrile with 0.1% formic acid at a flow rate of 400 nanoliters/minute (nL/min) with a column temperature of 55C. Data collection on the Fusion Lumos mass spectrometer was performed in a data-dependent acquisition (DDA) mode of acquisition with a r=120,000 (m/z 200) full MS scan from m/z 375 - 1500 with a target AGC value of 4e5 ions. MS/MS scans were acquired at Rapid scan rate (Ion Trap) with an AGC target of 1e4 ions and a max injection time of 100 ms. The total cycle time for MS and MS/MS scans was 2 sec. A 20 sec dynamic exclusion was employed to increase depth of coverage. The total analysis cycle time for each sample injection was approximately 1.5 hours. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-12-15 |
| AnnouncementXML | Submission_2025-12-15_11:19:47.604.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Erik Soderblom |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Phospho |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-15 11:09:24 | ID requested | |
| ⏵ 1 | 2025-12-15 11:19:48 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: label free, phosphoproteomics, DatasetType:Proteomics |
Contact List
| Albert R. La Spada | |
|---|---|
| contact affiliation | UC Irvine |
| contact email | alaspada@uci.edu |
| lab head | |
| Erik Soderblom | |
| contact affiliation | Duke University |
| contact email | es114@duke.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100225/ |
