⮝ Full datasets listing
PXD072012-1
PXD072012 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Protein-protein interactions of GTSF-1 and RRF-3 in C. elegans |
| Description | A mass spectrometry-based label-free proteomics analysis was performed to study the protein binders of GTSF-1 and RRF-3 in C. elegans. Anti-FLAG pull-downs were performed on 3xFLAG-tagged GTSF-1 (full-length or CTD-deleted) and 3xFLAG-tagged RRF-3 in C. elegans or in the wild-type N2 strain. Proteins were digested by trypsin. Peptides were measured on an Orbitrap Exploris 480 mass spectrometer. Raw data files were processed in DIA-NN software (version 2.2.0) in library-free mode. An in silico predicted spectral library was generated in DIA-NN from the UniProt reference proteomes of C. elegans (release 2024_03; 28,553 entries), E. coli K-12 strain (release 2024_03; 4,416 entries), the transgenic fusion proteins and a list of common contaminants. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-08-08 |
| AnnouncementXML | Submission_2026-08-08_02:45:11.361.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | J Chen |
| SpeciesList | scientific name: Caenorhabditis elegans; common name: roundworm; NCBI TaxID: 6239; |
| ModificationList | iodoacetamide - site C |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-15 07:41:14 | ID requested | |
| ⏵ 1 | 2026-08-08 02:45:11 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Protein-protein interactions, C. elegans, GTSF-1, RRF-3, DatasetType:Proteomics |
Contact List
| Rene Ketting | |
|---|---|
| contact affiliation | Institute of Molecular Biology, Mainz |
| contact email | R.Ketting@imb-mainz.de |
| lab head | |
| J Chen | |
| contact affiliation | Institute of Molecular Biology |
| contact email | j.chen@imb-mainz.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100222/ |
