PXD072001-1

PXD072001 is an original dataset announced via ProteomeXchange.

Title	N-terminal blocking sialic acid linkage specific alkylamidation for qualitative and quantitative analysis of sialytated glycoprotein
Description	Glycosylation is one of the most important post-translational modifications of proteins and plays a significant role in physiological progresses. Among monosaccharides, sialic acid has special functions due to its structure and position in glycans, and is related to diseases. There are multiple linkage isomers of sialic acids. It is difficult to distinguish them through LC-MS/MS because of their similar chromatographic behaviors and same mass. The SALSA reaction based on ring-opening ammonolysis has been widely applied in released N-glycans and N-glycopeptides, but we have found that reagents for derivatization would lead to condensation between peptides. Therefore, to address this issue, we introduced a strategy called N-SALSA, which involves blocking the N-terminal of the glycopeptide with acetaldehyde or acetaldehyde-13C2 before conducting the SALSA reaction. The optimized N-SALSA reaction has been well applied in standard glycopeptides and glycoprotein bovine fetuin. After 1:1 mixing of the isotopic diethyl labeled glycopeptides, we identified 106 differentially expressed intact N-glycopeptides from syphilis patient serum compared to healthy individuals, successfully achieving the site-specific distinguish of α2,6- and α2,3-linkage isomers through LC-MS/MS.
HostingRepository	iProX
AnnounceDate	2025-12-15
AnnouncementXML	Submission_2025-12-31_00:06:28.319.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wanwei Luo
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	complex glycosylation
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-15 01:36:15	ID requested
⏵ 1	2025-12-31 00:06:28	announced

Dataset with its publication pending

submitter keyword: stable isotopic diethyl labeling, sialic acid linkage specific alkylamidation, sialoglycopeptides, quantitative analysis

Zhixin Tian
contact affiliation	Tongji University
contact email	zhixintian@tongji.edu.cn
lab head
Wanwei Luo
contact affiliation	Tongji University
contact email	luowanwei@tongji.edu.cn
dataset submitter

iProX dataset URI