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PXD071978-1
PXD071978 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | RAS P21 Protein Activator 3 constrains Type I Immunity by limiting HCK kinase-mediated STAT4 phosphorylation |
| Description | Type I immunity, mediated by IFN-γ, is essential for combating intracellular pathogens but also drives inflammatory diseases. While the IL-12-STAT4 pathway that promotes IFN-γ production is well-established, the intrinsic negative regulators remain poorly defined. We identify RASA3 as a critical negative regulator whose expression is downregulated during Th1 cell differentiation. Its T cell-specific ablation amplified Th1/Tc1 responses, enhancing clearance of Listeria monocytogenes but exacerbating allergic contact dermatitis. Mechanistically, RASA3 constrains IFN-γ production by repressing the translation of the hematopoietic cell kinase (HCK) via ribosomal protein RPL36A. We further identify HCK as a novel direct kinase for STAT4, which binds to and phosphorylates it at Tyr693 residue. Additionally, the RASA3-HCK axis is conserved in human Th1 cells. Our work establishes RASA3 as a fundamental brake on type I immunity and a previously unrecognized role for HCK in STAT4 phosphorylation, presenting new potential targets for modulating Th1/Tc1-driven immune responses. |
| HostingRepository | iProX |
| AnnounceDate | 2025-12-12 |
| AnnouncementXML | Submission_2026-07-06_02:02:49.138.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jiayu Song |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-14 18:58:22 | ID requested | |
| ⏵ 1 | 2026-07-06 02:02:49 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: RASA3, HCK, STAT4, RPL36A |
Contact List
| Bing Wu | |
|---|---|
| contact affiliation | Wuhan University |
| contact email | bingwu@whu.edu.cn |
| lab head | |
| Jiayu Song | |
| contact affiliation | Wuhan University |
| contact email | 18326151404@139.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
