PXD071954-1

PXD071954 is an original dataset announced via ProteomeXchange.

Title	Effects of miRNA-30a 5p knock down in bone marrow CD71-positive erythroid precursor cells (imBMEP-A)
Description	Ex vivo production of red blood cells (RBCs) represents a promising alternative for transfusion medicine. Several strategies were described to generate erythroid cell lines from different sources, including embryonic, induced pluripotent and hematopoietic stem cells. With imBMEP-A, we have previously generated the first erythroid cell line directly derived from reticulocyte progenitors. However, the translation of cultured RBCs into clinical use is currently impeded by the inability to scale up to clinically meaningful doses primarily due to impaired in vitro enucleation of RBC progenitor cell lines in general. To identify factors contributing to the inefficient enucleation in immortalized RBC progenitor cell lines, we conducted a proteome analysis of the imBMEP cell line in comparison to hematopoietic stem cells (HSCs). For a detailed description of the cell culture condition, please see linked publication.
HostingRepository	PRIDE
AnnounceDate	2025-12-15
AnnouncementXML	Submission_2025-12-15_02:59:30.824.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristin Schubert
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-13 10:06:54	ID requested
⏵ 1	2025-12-15 02:59:31	announced

10.1007/S12015-025-10957-X;

Thiel J, S, ü, r, ü, n D, Br, ä, ndle DC, Teichert M, K, ü, nzel SR, Friedrich U, Dahl A, Schubert K, Rzagalinski I, Shevchenko A, Traikov S, Mirtschink P, Wagenf, ü, hr L, Buchholz F, H, ö, lig K, Tonn T, Kronstein-Wiedemann R, Knock Out of miRNA-30a-5p and Reconstitution of the Actin Network Dynamics Partly Restores the Impaired Terminal Erythroid Differentiation during Blood Pharming. Stem Cell Rev Rep, 21(8):2637-2653(2025) [pubmed]

10.1007/s12015-025-10957-x;

submitter keyword: mirNA-30A,erythropoesis, proteome

Kristin Schubert
contact affiliation	Department Molecular Toxicology, Helmholtz-Centre for Environmental Research GmbH (UFZ)
contact email	kristin.schubert@ufz.de
lab head
Kristin Schubert
contact affiliation	Brandenburg University of Technology Cottbus-Senftenberg
contact email	kristin.schubert@ufz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD071954

PRIDE project URI

• PRIDE
  1. PXD071954
     1. Label: PRIDE project
     2. Name: Effects of miRNA-30a 5p knock down in bone marrow CD71-positive erythroid precursor cells (imBMEP-A)