PXD071951-1

PXD071951 is an original dataset announced via ProteomeXchange.

Title	Thermo-sensing by mRNA with low-complexity and complementary untranslated sequences in trypanosomes
Description	Parasitic African trypanosomes experience temperature fluctuations due to fever symptomatic of infection, during developmental life cycle transitions in insect vectors and mammalian hosts, or due to diurnal shift. Since trypanosomes exhibit almost exclusive polycistronic transcription, gene expression regulation is dominated by post-transcriptional controls mediated by mRNA 3'-untranslated regions (3'-UTRs). Heat-shock responses have not been described in detail at the transcriptomic or proteomic level, however, and mechanisms underpinning thermo-sensing remain largely uncharacterised. We quantified >9,300 transcripts, and >5,900 proteins in bloodstream form Trypanosoma brucei after growth at 34°C, 37°C or 40°C for six hours. Increased protein abundance correlated with temperature for several hundred genes, encoding both the classical heat-shock proteins, and a second cohort including developmentally regulated genes. Transcript abundance also increased for genes close to transcription initiation sites, but this had minimal impact on protein abundance. Notably, the classical heat-shock response was characterised by both increased mRNA and protein abundance, while increased protein abundance dominated for the second cohort, suggesting translation control. Analysis of 3'-UTRs from post-transcriptionally regulated genes, using motif searching and machine learning, revealed enrichment of (UUA)4 motifs associated with heat-shock proteins, and enrichment of poly-purine and poly-pyrimidine tracts associated with translationally controlled genes. To determine whether these low-complexity sequences increased RNA secondary structure, we disrupted the RNA interference machinery and repeated transcriptomic profiling, essentially using argonaute as an in cellulo probe for native double-stranded RNA (dsRNA). As predicted, thermo-sensitive mRNAs displayed signatures of thermo-sensitive dsRNA formation. Thus, we provide transcriptomic and proteomic profiles of the heat-shock and thermo-sensing response in the African trypanosome. Thermo-sensitive UTRs are enriched in low-complexity and complementary sequences, supporting a post-transcriptional ‘zipper’ hypothesis whereby sequences that regulate mRNA stability and/or translation are progressively unmasked as temperature increases.
HostingRepository	PRIDE
AnnounceDate	2025-12-13
AnnouncementXML	Submission_2025-12-13_10:23:51.880.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michele Tinti
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: NEWT:5691;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-13 09:37:14	ID requested
⏵ 1	2025-12-13 10:23:52	announced

Dataset with its publication pending

submitter keyword: UTRs,Trypanosoma brucei

David Horn
contact affiliation	Biological Chemistry and Drug Discovery, School of Life Sciences, University od Dundee, Dundee
contact email	d.horn@dundee.ac.uk
lab head
Michele Tinti
contact affiliation	Dundee University
contact email	m.tinti@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD071951
     1. Label: PRIDE project
     2. Name: Thermo-sensing by mRNA with low-complexity and complementary untranslated sequences in trypanosomes