PXD071887 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Capture of candidate calpain5 interacting proteins from lysates of the human neuroblastoma SH-SY5Y cell line with analysis of LC-MSMS data using SWATH |
| Description | Calpain5 (CAPN5) is a nonclassical calpain in that it lacks a pentaEF hand domain. Although widely expressed dominantly inherited mutations of CAPN5 display phenotypes primarily localized to the eye. These experiments aimed to identify proteins from the neuroblastoma cell line SH-SY5Y that may interact with the catalytic core domain of CAPN5 (phe2-leu348) and to complement co-immunoprecipitation studies using SH-SY5Y cell overexpressing full length CAPN5. Given that domains are independent folding units within the intact protein and interacting surfaces are in general localized some valid interacting proteins are expected to be captured. It is also clear that some interacting partners may be lost due to a decreased number of, or affinity of, interactions.. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-02 |
| AnnouncementXML | Submission_2026-02-01_16:29:04.414.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Carlos Gartner |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-12-11 14:25:57 | ID requested | |
| ⏵ 1 | 2026-02-01 16:29:05 | announced | |
Publication List
| Gal J, Bondada V, Crasta R, Croall DE, Vary CP, Geddes JW, Candidate Interaction Partners of Calpain-5 Suggest Clues to Its Involvement in Neovascular Inflammatory Vitreoretinopathy. Cells, 15(2):(2026) [pubmed] |
| 10.3390/cells15020142; |
Keyword List
| ProteomeXchange project tag: Human Proteome Project |
| submitter keyword: SH-SY5Y cell line, LC-MSMS, catalytic domain, SWATH, human,Calpain5 |
Contact List
| Calvin Vary |
|---|
| contact affiliation | Director, Proteomics and Lipidomics Core Facility, Mainehealth |
| contact email | Calvin.Vary@mainehealth.org |
| lab head | |
| Carlos Gartner |
|---|
| contact affiliation | MaineHealth Institute for Research |
| contact email | gusti.gartner@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071887
- Label: PRIDE project
- Name: Capture of candidate calpain5 interacting proteins from lysates of the human neuroblastoma SH-SY5Y cell line with analysis of LC-MSMS data using SWATH
