PXD071864-1

PXD071864 is an original dataset announced via ProteomeXchange.

Title	Proteome of Cas9- and dCas9-expressing Lucilia cuprina larvae reared on vs. off doxycycline
Description	We analyzed the proteomes of transgenic Cas9- and dCas9-expressing larvae of the Australian sheep blowfly, Lucilia cuprina. We measured abundance of red fluorescent protein (dsRed) in our samples in order to test Cas9 and dCas9 activity. Flies from two transgenic strains, DR7#2;Cas9-ryt and DR7#2;dCas9-ryt/+, were reared on or off 25 ug/mL doxycycline. Removal of doxycycline from the diet of these transgenic flies should activate the expression of Cas9 or dCas9 genes. Flies reared on doxycycline served as our control groups, as these flies should express no (or minimal amounts of) Cas9 or dCas9. Four replicate samples were obtained from each experimental and control group, for a total of 16 samples across this experiment. Each replicate consisted of a single third instar (wandering stage) larvae. Whole-tissue samples were flash-frozen in liquid nitrogen and stored at -80°C prior to sample preparation for 1D-LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-27_12:56:42.347.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olivia Mathieson
SpeciesList	scientific name: Lucilia cuprina; NCBI TaxID: NEWT:7375;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-11 00:49:57	ID requested
⏵ 1	2026-04-27 12:56:42	announced

10.1177/25731599261436373;

submitter keyword: insect, Blowfly, Diptera, TET-OFF,1D-LC, dCas9, Cas9, Orbitrap Astral, Calliphoridae, DDA, Lucilia

Manuel Kleiner
contact affiliation	North Carolina State University
contact email	manuel_kleiner@ncsu.edu
lab head
Olivia Mathieson
contact affiliation	Department of Plant and Microbial Biology, North Carolina State University
contact email	olmathie@ncsu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD071864
     1. Label: PRIDE project
     2. Name: Proteome of Cas9- and dCas9-expressing Lucilia cuprina larvae reared on vs. off doxycycline