PXD071840-1

PXD071840 is an original dataset announced via ProteomeXchange.

Title	Skin protection against UVA induced changes in cells proteome and intercellular signaling by the 3-O-ethyl ascorbic acid
Description	UVA radiation is one of the main harmful factor that penetrates all human skin layers and influencing communication between the cells that build them. To prevent UVA-induced damage, there is a constant search for compounds that could protect all skin cells, as well as restoring homeostasis in their communication. Therefore, the aim of this study was to evaluate the effect of 3-O-ethyl ascorbic acid (EAA) on the intracellular proteome of co-cultured keratinocytes and fibroblasts after UVA irradiation and on the profile of proteins released into the medium by both cell types. Proteomic approach allowed to identify significantly modified by UVA as well as EAA proteins. In keratinocytes UVA radiation enhanced expression of pro-inflammatory and pro-proliferative/keratinizing proteins and decreased anti-apoptotic and antioxidant proteins, while in fibroblasts UVA radiation induced expression mainly of proinflammatory proteins, simultaneously decreasing the level of proteins involved in antioxidant response, as well as growing factors. Similar effect was observed in the medium of co-cultured cells, where increase in pro-inflammatory protein and decrease in growing factors were observed. Medium supplementation with EAA restored the levels of these proteins compared to control cultures. The results of this study show that EAA may act as a protector of epidermal and dermal cells, because it reduces the levels of pro-inflammatory proteins and increases the activity of the antioxidant system of skin cells (keratinocytes and fibroblasts), as well as normalizes intercellular signaling.
HostingRepository	PRIDE
AnnounceDate	2026-08-24
AnnouncementXML	Submission_2026-08-23_16:24:30.492.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Agnieszka Gęgotek
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-10 17:44:17	ID requested
⏵ 1	2026-08-23 16:24:31	announced

10.3390/ijms27125551;

submitter keyword: skin cells

3-O-ethyl ascorbic acid

co-culture

proteomics

UVA radiation

Agnieszka Gegotek
contact affiliation	UMB
contact email	agnieszka.gegotek@umb.edu.pl
lab head
Agnieszka Gęgotek
contact affiliation	Department of Analytical Chemistry, Medical University of Bialystok, Mickiewicza 2D, 15-222 Bialystok, Poland
contact email	agnieszka.gegotek@umb.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD071840

PRIDE project URI

• PRIDE
  1. PXD071840
     1. Label: PRIDE project
     2. Name: Skin protection against UVA induced changes in cells proteome and intercellular signaling by the 3-O-ethyl ascorbic acid