PXD071833-1

PXD071833 is an original dataset announced via ProteomeXchange.

Title	DIA proteomic profiling of psoriasis: pharmacological rescue in an imiquimod-induced mouse model and CRISPR knockout human keratinocytes
Description	This dataset contains quantitative, bottom-up DIA proteomics data generated as part of a larger study entitled "AI-Guided CRISPR Screen Reveals Repurposed Drugs as Topical Psoriasis Therapeutics," and is organized into two complementary sub-studies. Sub-study 1 – Pharmacological Rescue in a Mouse Psoriasis Model The first proteomics component was designed to molecularly characterize pharmacological rescue effects in an imiquimod (IMQ)-induced mouse psoriasis model following prioritization of candidate targets from a CRISPR-based genetic screen. Mouse skin samples were collected from five experimental conditions: untreated control, IMQ only, IMQ plus the ALOX5 inhibitor zileuton, IMQ plus the OXTR antagonist cligosiban, and IMQ plus an IL-17RA blocking antibody serving as a positive control. Skin tissues were processed for bottom-up proteomics and analyzed using dia-PASEF on a timsTOF Ultra 2 mass spectrometer coupled to an Evosep One liquid chromatography system. Proteomic data were analyzed using DIA-NN in library-free mode against a SwissProt-reviewed Mus musculus reference proteome (Mouse_SwissProt_CanonicalAndIsoform_20250324.fasta). The resulting protein-level quantitative dataset enables assessment of molecular pathways associated with IMQ-driven inflammation and the extent to which these signatures are modulated or rescued by pharmacological intervention. Sub-study 2 – CRISPR Knockout Proteomics in Human Keratinocytes The second proteomics component was designed to characterize the proteomic consequences of genetically ablating the candidate therapeutic targets ALOX5 and OXTR in a human cellular psoriasis-relevant context. Human keratinocytes were subjected to CRISPR-mediated knockout of either ALOX5 or OXTR and compared against wild-type controls. Samples were processed for bottom-up proteomics and analyzed using dia-PASEF on a timsTOF Ultra 2 mass spectrometer coupled to an Evosep One liquid chromatography system. Proteomic data were analyzed using DIA-NN in library-free mode against a SwissProt-reviewed Homo sapiens reference proteome. The resulting dataset enables assessment of the downstream molecular signatures driven by loss of each target in human keratinocytes.
HostingRepository	PRIDE
AnnounceDate	2026-05-21
AnnouncementXML	Submission_2026-05-21_12:13:02.840.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ryan McClure
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Ultra 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-10 14:21:20	ID requested
⏵ 1	2026-05-21 12:13:03	announced

Dataset with its publication pending

submitter keyword: None

Ryan McClure
contact affiliation	MS Omics, Biohub Chicago, USA
contact email	ryan.mcclure@biohub.org
lab head
Ryan McClure
contact affiliation	Biohub Chicago
contact email	ryan.mcclure@biohub.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD071833

PRIDE project URI

• PRIDE
  1. PXD071833
     1. Label: PRIDE project
     2. Name: DIA proteomic profiling of psoriasis: pharmacological rescue in an imiquimod-induced mouse model and CRISPR knockout human keratinocytes