PXD071831-1

PXD071831 is an original dataset announced via ProteomeXchange.

Title	Life-Death Trade-offs: HSV-1 ICP27 as a Cellular Context-Dependent Regulator of Intrinsic Apoptosis
Description	Herpes simplex virus type 1 (HSV-1) infection represents an established model for studying virus-host interactions, given its ability to modulate cell fate and achieve long-term persistence. Among the immediate early genes, the multifunctional regulatory protein ICP27, encoded by the UL54 gene, plays a pivotal role in coordinating viral gene expression and remodeling host signaling pathways. This study investigates the role of ICP27 in modulating the intrinsic apoptotic pathway in two cell models representative of different stages of infection: hTert RPE-1 epithelial cells, modeling the lytic replication, and SH-SY5Y neuroblastoma cells, mimicking a neuron-like microenvironment. To evaluate the specific function of HSV-1 ICP27 in this field, cells infected with wild-type HSV-1 (HSV-1 w.t.) were compared with a viral vector lacking ICP27 (HSV-1ΔICP27). Analyses were conducted using RT-qPCR for gene quantification, western blotting for protein evaluation, enzymatic assays to measure caspase activity, flow cytometry to determine reactive oxygen species (ROS) production and mitochondrial functionality, and quantitative proteomic approaches for the global characterization of infection-induced protein changes. ICP27 exerts cell type-dependent effects on apoptosis. In epithelial cells, ICP27 promotes programmed cell death by activating caspase-9 and effector caspases (3/7), increasing reactive oxygen species (ROS) generation, altering mitochondrial function, and elevating the Bax/Bcl-2 ratio, indicative of mitochondrial pathway activation. Conversely, in neuron-like cells, ICP27 suppresses apoptotic signaling by reducing caspase activity, limiting oxidative stress, and preserving mitochondrial integrity. These findings position ICP27 as a cellular context-dependent regulator of apoptosis, driving lytic apoptosis in epithelial cells while favoring host cell survival in neuronal environments. This dual behavior underscores HSV-1 adaptability across microenvironments, with implications for viral pathogenesis and therapeutic targeting.
HostingRepository	PRIDE
AnnounceDate	2026-06-16
AnnouncementXML	Submission_2026-06-16_00:40:00.691.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Davide Barboni
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-10 13:26:55	ID requested
⏵ 1	2026-06-16 00:40:01	announced

10.3389/FMICB.2026.1849379;

submitter keyword: HSV-1, ICP27, hTert RPE-1, SH-SY5Y, apoptosis

Martina Catani
contact affiliation	Dipartimento di Scienze Chimiche, Farmaceutiche ed Agrarie, Università degli Studi di Ferrara
contact email	ctnmtn@unife.it
lab head
Davide Barboni
contact affiliation	Università degli Studi di Ferrara
contact email	brbdvd1@unife.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD071831

PRIDE project URI

• PRIDE
  1. PXD071831
     1. Label: PRIDE project
     2. Name: Life-Death Trade-offs: HSV-1 ICP27 as a Cellular Context-Dependent Regulator of Intrinsic Apoptosis