PXD071811-1

PXD071811 is an original dataset announced via ProteomeXchange.

Title	A Novel Human Cellular System for Studying Normal Aging and for Anti-Aging Discovery
Description	Aging studies using animal and cellular models have uncovered key proteins and pathways central to organismal aging. However, these models differ genetically and physiologically from human aging, posing challenges in translating discoveries to human contexts. In this study, we present a human normal cell aging model based on the development of cytotrophoblasts (CTBs) to syncytiotrophoblasts (STBs) in the placenta. The in vitro-derived STBs from human trophoblast stem cells (hTSCs) recapitulate the maturation and major cellular aging features of in vivo CTB-STB, including multinucleation, hormone secretion, cell cycle arrest, genome instability, epigenetic changes, activation of endogenous transposable elements, and senescence-associated secretory phenotypes (SASPs). Notably, the progressive senescence in the trophoblast system closely matches the predicted aging trajectory of other human tissue stem cells. Known anti-aging molecules, such as mTOR inhibitors and senolytics, attenuate senescence signals in STBs. The established CGA-EGFP reporter hTSC line enables scalable and quantitative screening and identified candidates with it can be further extended to other context-specific aging processes like that of skin fibroblasts. The hTSC-STB system represents a novel physiologically accelerated cellular aging model, bridges the gap between fundamental aging research and interventions, and prioritizes anti-aging candidates for clinical development.
HostingRepository	PRIDE
AnnounceDate	2026-01-26
AnnouncementXML	Submission_2026-01-25_21:34:25.542.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhen feng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-10 05:15:26	ID requested
⏵ 1	2026-01-25 21:34:26	announced

10.1111/acel.70352;

Feng Z, Li CS, Fu H, Jiang W, Zhang W, Huang Y, Huang Y, Tam TTKK, Li Y, Liu F, Lu L, Lee YL, Yeung WSB, Dougan G, Liu P, A Novel Human Cellular System for Studying Normal Aging and for Anti-Aging Discovery. Aging Cell, 25(2):e70352(2026) [pubmed]

submitter keyword: Aging, Senescence, Syncytiotrophoblasts (STBs), Trophoblast stem cells (hTSCs)

Prof. Pengtao Liu
contact affiliation	InnoHK Centre for Translational Stem Cell Biology, The University of Hong Kong
contact email	pliu88@hku.hk
lab head
zhen feng
contact affiliation	Centre for Translational Stem Cell Biology 幹細胞轉化研究中心
contact email	zhen.feng@hkstemcell.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD071811

PRIDE project URI

• PRIDE
  1. PXD071811
     1. Label: PRIDE project
     2. Name: A Novel Human Cellular System for Studying Normal Aging and for Anti-Aging Discovery