PXD071796-1

PXD071796 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of gonadal tissue in Solea senegalensis reveals reproductive deregulation associated with F1 individuals
Description	Reproductive dysfunction in captive-bred Senegalese sole (Solea senegalensis) limits the consolidation of its aquaculture production, particularly due to reduced fertility and poor sperm quality in F1 males. To elucidate the molecular mechanisms underlying this problem, a quantitative proteomic analysis was conducted on gonadal tissues from wild and F1 males and females using LC–MS/MS. A total of 2,221 proteins were identified, of which 1,797 were retained after quality filtering. Comparative analyses revealed a clear segregation by origin (F1(cultivated) and wild) and sex (male and female), and 86 proteins were differentially expressed between F1 and wild males. Functional enrichment showed a significant downregulation of key reproductive processes in F1 males, including sperm–egg recognition, binding of sperm to zona pellucida, and acrosome reaction, suggesting impaired gamete interaction and fertilization ability. Conversely, F1 males displayed enrichment of metabolic and proteolytic pathways, indicative of compensatory energy demands. Protein–protein interaction network analysis identified a reproductive subnetwork dominated by zona pellucida sperm-binding proteins, which exhibited reduced connectivity in F1 males. These results demonstrate a coordinated suppression of molecular components essential for sperm–egg communication and acrosomal exocytosis, providing proteomic evidence for a systemic deregulation of the reproductive machinery in F1 fish. This study identifies potential protein biomarkers linked to reproductive performance, offering molecular targets to improve broodstock management and fertilization success in S. senegalensis aquaculture.
HostingRepository	PRIDE
AnnounceDate	2026-08-24
AnnouncementXML	Submission_2026-08-23_16:31:24.667.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marco Eliecer Anaya Romero
SpeciesList	scientific name: Solea senegalensis; NCBI TaxID: NEWT:28829;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-09 19:52:37	ID requested
⏵ 1	2026-08-23 16:31:25	announced

Anaya-Romero M, Arias-P, é, rez A, Ram, í, rez D, Rodr, í, guez ME, Merlo MA, Portela-Bens S, Cross I, Robledo D, Rebordinos L, . Int J Mol Sci, 27(5):(2026) [pubmed]

10.3390/ijms27052153;

ProteomeXchange project tag: Bioinformatics Infrastructure for Life Sciences (BILS) network (Sweden)

submitter keyword: Solea senegalensis

gonadal tissue

proteomic analysis

reproductive dysfunction.

Marco Eliecer Anaya Romero
contact affiliation	Universidad Simón Bolívar, Facultad de Ciencias Básicas y Biomédicas, Centro de Investigaciones en Ciencias de la Vida, Barranquilla, Colombia
contact email	marco.anaya@unisimon.edu.co
lab head
Marco Eliecer Anaya Romero
contact affiliation	Universidad Simón Bolívar, Facultad de Ciencias Básicas y Biomédicas, Centro de Investigaciones en Ciencias de la Vida, Barranquilla, Colombia
contact email	marco.anaya@unisimon.edu.co
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD071796

PRIDE project URI

• PRIDE
  1. PXD071796
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of gonadal tissue in Solea senegalensis reveals reproductive deregulation associated with F1 individuals