⮝ Full datasets listing
PXD071787-1
PXD071787 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The Use of Multi-species Probiotics derived Vesicles to Encapsulate Luteolin for Preventing Colitis in a Mouse Model |
| Description | Probiotic-derived vesicles were suggested to exhibit comparable biological effects to those of living probiotics. Given their lower immunogenic properties, these vesicles may serve as carriers for enhancing the bioavailability of chemical components utilized in dietary intervention. However, molecular compositions and biological effects of probiotic-derived vesicles may vary depending on probiotic cultural conditions. Luteolin (Lut), a hydrophobic flavonoid, has been shown to possess antioxidant and anti-inflammatory properties in mouse colitis models. In this study, we have produced membrane vesicles (MVs) either from a single strain of probiotic (S-MVs) or co-cultured multiple symbiotic probiotics (M-MVs). Notably, M-MVs exhibited superior free radical scavenging ability compared to each of the S-MVs, attributed to the presence of characteristic anti-oxidation proteins in M-MVs. Subsequently, Lut was loaded into M-MVs, in generating Lut-M-MVs (Lut-MVs). Lut-MVs demonstrated enhanced water solubility and gastrointestinal stability relative to Lut alone, and exhibited improved anti-oxidative capacity compared to M-MVs. The prevention potential of Lut-MVs was investigated in a dextran sulfate sodium (DSS)-induced mouse colitis model. Our results indicated that Lut-MVs prevented colitis not only by alleviating oxidative damage but also improving intestinal barrier function and reducing inflammatory response. Additionally, Lut-MVs were found to regulate microbial balance in the colon. This study provides a novel method for preparing probiotic-derived vesicles and highlights their significant potential for delivering hydrophobic drugs in the treatment of colon diseases. |
| HostingRepository | iProX |
| AnnounceDate | 2025-12-09 |
| AnnouncementXML | Submission_2026-05-06_18:22:13.021.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Haishan Yang |
| SpeciesList | scientific name: Bifidobacterium longum 3_1_37DFAAB; NCBI TaxID: 1000567; scientific name: Lactobacillus reuteri; NCBI TaxID: 1598; scientific name: Clostridium butyricum; NCBI TaxID: 1492; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-09 17:36:43 | ID requested | |
| ⏵ 1 | 2026-05-06 18:22:14 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: MVs, luteolin, IBD |
Contact List
| Shulin Hou | |
|---|---|
| contact affiliation | Department of Biochemistry and Molecular Biology, Shanxi Medical University |
| contact email | hou_shulin1@163.com |
| lab head | |
| Haishan Yang | |
| contact affiliation | Department of Biochemistry and Molecular Biology, Shanxi Medical University |
| contact email | 1739884541@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
