PXD071742 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterizing the Effects of Protein Glycosylation Perturbation on Phosphorylation Signaling |
| Description | This study investigates how engineered N-glycan remodeling affects phosphorylation signaling networks in human cells. We generated a glycoengineered HEK293 cell line (AI) with eliminated core fucosylation, enhanced sialylation, and reduced GlcNAc branching through sequential CRISPR/Cas9 knockouts (FUT8, MGAT4A/B, MGAT5, GMDS) and knock-in of sialuria-associated GNE mutations (R263L/R266Q), combined with stable overexpression of ST6Gal1, B4GalT1, MGAT1, and MGAT2. Both wildtype (WT) and AI cells were subjected to three treatment conditions in biological triplicate: normal growth in complete medium, 12-hour serum starvation, and 12-hour starvation followed by 30-minute serum re-stimulation (10% FBS). We performed integrated multi-omics profiling using TMT18-based quantitative proteomics to simultaneously characterize the global proteome (24 fractions), phosphoproteome (12 Fe-IMAC enriched fractions), and glycoproteome (12 MAX-enriched fractions) across all conditions. This dataset enables systematic interrogation of glycan-phosphorylation crosstalk and identifies glycan-dependent signaling rewiring in response to growth factor stimulation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-09 |
| AnnouncementXML | Submission_2026-09-09_05:54:06.425.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hongyi Liu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | N-glycosylated residue; phosphorylated residue |
| Instrument | timsTOF HT; Orbitrap Ascend |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-12-08 19:37:14 | ID requested | |
| ⏵ 1 | 2026-09-09 05:54:07 | announced | |
Publication List
| Wei E, Liu H, Betenbaugh M, Zhang H, Characterizing the Effects of Protein Glycosylation Perturbation on Phosphorylation Signaling. Anal Chem, 98(25):18577-18595(2026) [pubmed] |
| 10.1021/acs.analchem.6c00790; |
Keyword List
| submitter keyword: proteomics,glycosylation, phosphorylation, serum starvation, serum stimulation, glycoengineering |
Contact List
| Hui Zhang |
|---|
| contact affiliation | Johns Hopkins University |
| contact email | huizhang@jhu.edu |
| lab head | |
| Hongyi Liu |
|---|
| contact affiliation | Johns Hopkins University |
| contact email | hliu173@jh.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071742
- Label: PRIDE project
- Name: Characterizing the Effects of Protein Glycosylation Perturbation on Phosphorylation Signaling
