PXD071722-1

PXD071722 is an original dataset announced via ProteomeXchange.

Title	Granulated bovine oocytes represent a lipid rich phenotype with enhanced developmental competence
Description	Immature bovine cumulus-oocytes complexes (COCs) with several layers of tightly packed cumulus cells and a homogeneous, darkish ooplasm are considered to be of good quality, whereas COCs exhibiting cytoplasmatic granulation are frequently observed but routinely discarded. This study compared the protein and lipid landscape, as well as the developmental competence, of immature cattle oocytes with either granulated or homogeneous ooplasm but similarly compact cumulus investment. Nile Red staining revealed increased lipid droplet accumulation, greater heterogeneity in droplet size, and formation of droplet clusters in granulated oocytes. Lipidomics via ESI-IT MS corroborated these findings by discovering a significantly higher content of phosphatidylcholines and a trend toward increased levels of free fatty acids in granulated compared to homogeneous oocytes. Protein profiling via LC-MS/MS was performed to characterize the molecular features of granulated oocytes in more detail. In total, 2136 proteins were detected, with only minor differences between the proteomes of granulated and homogenous oocytes. All ten proteins classified as differentially expressed displayed a lower abundance in granulated oocytes - seven were directly associated with peroxisomal lipid metabolism and peroxisome formation, mitochondrial function, and autophagy initiation. Protein interaction analysis revealed methyl-branched fatty acid metabolic process and fatty acid alpha-oxidation as enriched pathways with the highest strength. Granulated oocytes displayed significantly higher cleavage and blastocyst formation rates following in vitro embryo production than homogeneous oocytes, indicating superior developmental competence. Granulated oocytes thus represent a distinct, lipid‑rich phenotype with configured peroxisomal and mitochondrial pathways that, contrary to current selection practice, can enhance early embryonic development.
HostingRepository	PRIDE
AnnounceDate	2026-05-27
AnnouncementXML	Submission_2026-05-27_09:00:28.024.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Falk Butter
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-08 10:14:18	ID requested
⏵ 1	2026-05-27 09:00:28	announced

Herbicht R, Engel KM, Teschke L, Butter F, Fr, ö, hlich T, Klein C, Granulated bovine oocytes represent a lipid rich phenotype with enhanced developmental competence. Anim Reprod Sci, 292():108237(2026) [pubmed]

10.1016/j.anireprosci.2026.108237;

submitter keyword: LC-MS/MS

Falk Butter
contact affiliation	Friedrich-Loeffler-Institute
contact email	falk.butter@fli.de
lab head
Falk Butter
contact affiliation	IMVZ, Friedrich-Loeffler-Institute
contact email	falk.butter@fli.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD071722
     1. Label: PRIDE project
     2. Name: Granulated bovine oocytes represent a lipid rich phenotype with enhanced developmental competence