PXD071605 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | An N-Acetyltransferase-MAPK fusion protein modulates developmental reprogramming in Physcomitrium patens |
| Description | Post-translational modifications (PTMs) finetune plant responses to developmental and environmental cues by impacting protein activity, stability, localization and interaction landscape. In this study we identified a moss specific protein which combines two common PTMs: acetylation and phosphorylation. This protein originated from the fusion of a MAPK with an N-acetyltransferase, for which we named it Rosetta NATD-MAPK 1 (RAK1). Using biochemical methods, we demonstrated that RAK1 has acetyltransferase activity that is enhanced by activation of its MAPK domain. Phenotypical studies of rak1 knockout mutants revealed a role for RAK1 in the regulation of the 2D-to-3D growth transition. Through mass spectrometry, we identified differential acetylation and phosphorylation events associated with metabolic reprogramming and gametophore formation in the mutants. Additional experiments also revealed that RAK1 interacts with proteins associated to several biological processes, including metabolism. Collectively, this study uncovers a previously unknown multidomain protein and provides mechanistic insights into the interplay of PTMs during developmental reprogramming. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-22 |
| AnnouncementXML | Submission_2026-04-22_02:22:04.048.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Richard Imre |
| SpeciesList | scientific name: Physcomitrella patens subsp. patens (Moss); NCBI TaxID: NEWT:3218; |
| ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-12-04 19:33:06 | ID requested | |
| ⏵ 1 | 2026-04-22 02:22:04 | announced | |
Publication List
Keyword List
| submitter keyword: 3D growth, NATD, development, MAPK,Physcomitrium patens, RAK1 |
Contact List
| Morten Petersen |
|---|
| contact affiliation | University of Copenhagen, Department of Plant and Environmental Sciences |
| contact email | morten.petersen@plen.ku.dk |
| lab head | |
| Richard Imre |
|---|
| contact affiliation | IMBA Vienna |
| contact email | richard.imre@imba.oeaw.ac.at |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071605
- Label: PRIDE project
- Name: An N-Acetyltransferase-MAPK fusion protein modulates developmental reprogramming in Physcomitrium patens
