PXD071603 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Dysregulation of Non-Muscle Myosin IIA Assembly and Phosphorylation in S100A4 Null Mouse Lens |
| Description | Non-muscle myosin IIA (NMIIA), a motor protein plays a critical role in regulating cell morphology, adhesion, migration, contractility, and mechanotransduction across various tissues, including the ocular lens. S100A4, a known NMIIA-interacting protein, is abundantly expressed in lens fibers and shows discrete spatial distribution. Loss of S100A4 has previously been shown to associate with late-onset lens opacification in mice. However, its role in regulating NMIIA activity, assembly, and actin cytoskeletal organization in the lens remains unclear. Using S100A4-null mice, this study reveals that S100A4 co-immunoprecipitates with NMIIA and that its absence leads to decreased NMIIA (Ser1943) phosphorylation, impaired NMIIA filament assembly, and disruption in actin cytoskeletal organization and polymerization in lens. Quantitative proteomic analysis of cytoskeletal- and membrane-enriched fractions from S100A4-null versus wild-type lenses further identified decreased levels of CLIC5 and RNA binding protein-SERBP1. Moreover, treatment of wild-type mouse lenses in ex-vivo with trifluoperazine, a known S100A4 inhibitor, induced lens opacification in association with increased insolubilization of S100A4, NMIIA and actin. Collectively, these findings demonstrate that S100A4 plays a critical role in regulating NMIIA activity and assembly and maintaining actin cytoskeletal organization in the ocular lens, thereby contributing to lens transparency and homeostasis. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-29 |
| AnnouncementXML | Submission_2026-06-28_16:36:48.497.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD071603 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Nik Skiba |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-12-04 18:38:42 | ID requested | |
| ⏵ 1 | 2026-06-28 16:36:49 | announced | |
Publication List
| 10.6019/PXD071603; |
| 10.1002/jcp.70202; |
| Maddala R, Lankford LK, Skiba NP, Rao PV, Dysregulation of Non-Muscle Myosin IIA Assembly and Phosphorylation in S100A4 Null Mouse Lens. J Cell Physiol, 241(6):e70202(2026) [pubmed] |
Keyword List
| submitter keyword: mouse, actin cytoskeleton, cataract, S1004A, Myosin IIA, lens |
Contact List
| Ponugoti V. Rao |
|---|
| contact affiliation | Duke University Eye Center |
| contact email | p.rao@duke.edu |
| lab head | |
| Nik Skiba |
|---|
| contact affiliation | Duke University |
| contact email | skibanik@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071603
- Label: PRIDE project
- Name: Dysregulation of Non-Muscle Myosin IIA Assembly and Phosphorylation in S100A4 Null Mouse Lens
