PXD071600-1

PXD071600 is an original dataset announced via ProteomeXchange.

Title	Systematic discovery of potential regulators of transcriptional variability reveals NAP1L1 as a transcriptional stabilizer in mouse ESCs
Description	Embryonic stem cells (ESCs) exhibit gene expression heterogeneity of key pluripotency factors (e.g. Nanog), suggesting functional relevance. To systematically identify potential regulators of transcriptional variability, we integrated single-cell RNA-seq (scRNA-seq) with ChIP-seq datasets in ESCs. One of the potential ‘stabilizer’ proteins we identified is Nucleosome Assembly Protein 1-Like 1 (NAP1L1), a member of the NAP1 family. To directly test the function of NAP1L1 as a transcriptional stabilizer, we first recruited NAP1L1 to the Nanog promoter via fusion with dCas9. NAP1L1 recruitment stabilized Nanog expression in ESCs. To further explore NAP1L1’s activity genome-wide, we analyzed transcriptional heterogeneity in WT and Nap1l1-knock-out (KO) ESCs using scRNA-seq, revealing increased transcriptional variability in Nap1l1-KO ESCs. Finally, co-IP of NAP1L1 followed by Mass-Spectrometry (LC-MS/MS) identified DPPA3 as a key interacting partner of NAP1L1. Taken together, we provide a systematic discovery platform for regulators of transcriptional variability and identify NAP1L1 as a transcriptional stabilizer in ESCs.
HostingRepository	PRIDE
AnnounceDate	2026-08-06
AnnouncementXML	Submission_2026-08-06_14:50:47.293.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Segal
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-04 17:04:06	ID requested
⏵ 1	2026-08-06 14:50:47	announced

Dataset with its publication pending

submitter keyword: mESC

NAP1L1

DPPA3

Transcriptional variability

Pluripotency

Tamar Segal
contact affiliation	Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem, Israel
contact email	tamar.segal@mail.huji.ac.il
lab head
Tamar Segal
contact affiliation	The Hebrew University
contact email	tamar.segal@mail.huji.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD071600

PRIDE project URI

• PRIDE
  1. PXD071600
     1. Label: PRIDE project
     2. Name: Systematic discovery of potential regulators of transcriptional variability reveals NAP1L1 as a transcriptional stabilizer in mouse ESCs