PXD071582-1

PXD071582 is an original dataset announced via ProteomeXchange.

Title	Robust Neurogenesis in Chronic Stroke Monkeys Following Mesenchymal Stem Cell Transplantation Plus Intermittent Theta-burst Stimulation
Description	A significant population of chronic stroke patients urgently requires more effective therapies due to functional plateaus and limited intervention options. Intermittent theta-burst stimulation (iTBS) has been increasingly adopted in chronic-phase ischemic stroke management. However, the efficacy decreases over time. Mesenchymal stem cells (MSCs) have emerged as a promising therapeutic strategy for chronic stroke management through multiple mechanisms. Whether combined MSC transplantation and iTBS confers superior synergistic efficacy warrants further investigation. In this study, human umbilical cord-derived MSCs were stereotactically injected into the penumbra of the brain parenchyma adjacent to infarct sites in cynomolgus monkeys with chronic-stage middle cerebral artery occlusion (MCAO)-induced ischemic stroke. iTBS commenced at 1 week post-transplantation (5 sessions/week until week 17). Behavioral tests, electrophysiological recordings, functional magnetic resonance imaging (fMRI), magnetic resonance spectroscopy (MRS), and plasma proteomic analysis were conducted longitudinally, with terminal histological and spatial proteomic analysis at week 17. The results demonstrated that combined MSC/iTBS therapy enhanced motor function, reduced cortical excitation thresholds, shortened motor-evoked potential (MEP) latency, increased neural activity intensity/synchronization, strengthened functional connectivity, and optimized motor cortex metabolism. In addition to the commonly attributed therapeutic mechanisms of MSCs and iTBS, the combined therapy additionally triggered neurogenesis and stem cell chemotaxis, which was potentially mediated by iTBS-enhanced CXCL12 secretion from MSCs. Therefore, MSC/iTBS combination therapy can effectively treat chronic stroke through multiple mechanisms, particularly involving the activation of endogenous neural stem cells (NSCs), representing a promising interdisciplinary treatment strategy.
HostingRepository	PRIDE
AnnounceDate	2026-03-12
AnnouncementXML	Submission_2026-03-12_02:25:30.831.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuan-Huan Ma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Macaca mulatta (Rhesus macaque); NCBI TaxID: NEWT:9544;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics timsTOF series; Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-04 10:33:29	ID requested
⏵ 1	2026-03-12 02:25:31	announced

Dataset with its publication pending

submitter keyword: Chronic stroke

Nonhuman primate (NHPs)

Mesenchymal stem cells (MSCs)

Transplantation

Intermittent theta-burst stimulation (iTBS)

Neuromodulation

Neurogenesis

Yue Lan
contact affiliation	Department of Rehabilitation Medicine, The Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou 510180, China
contact email	bluemooning@163.com
lab head
Yuan-Huan Ma
contact affiliation	Sun Yat-sen University
contact email	mayuanh@foxmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD071582

PRIDE project URI

• PRIDE
  1. PXD071582
     1. Label: PRIDE project
     2. Name: Robust Neurogenesis in Chronic Stroke Monkeys Following Mesenchymal Stem Cell Transplantation Plus Intermittent Theta-burst Stimulation