PXD071560-1

PXD071560 is an original dataset announced via ProteomeXchange.

Title	A Pore-Forming Toxin Monalysin Contributes to Infection-Induced Suppression of Defecation in Female Drosophila
Description	Pathogen expulsion from the gut via defecation is an important defence strategy against infection. The microbial factors that can subvert this defence reaction remain poorly understood. While many microbes have been found to increase host intestinal peristalsis, Pseudomonas entomophila infection in Drosophila melanogaster leads to infection-induced defecation blockage, particularly in females. Here, we show that this phenotype is driven by a secreted, thermosensitive protein regulated by the GacS/GacA two-component system. Proteomic comparison of the ΔgacA mutant, which does not inhibit defecation, and the avirulent Δhfq mutant lacking RNA chaperon Hfq, which still triggers the phenotype, identified pore-forming toxin Monalysin as one of the candidate factors required for inhibiting defecation. Consistently, the Monalysin-deficient mutant was unable to inhibit defecation. Hence, Monalysin besides causing intestinal damage, has a previously unknown role in suppressing defecation and potentially pathogen expulsion. Overall, our study identified a bacterial factor that rapidly reduces defecation frequency, consistent with transient suppression of intestinal transit, thus advancing our understanding of pathogen strategies used to subvert host defences.
HostingRepository	PRIDE
AnnounceDate	2026-08-18
AnnouncementXML	Submission_2026-08-18_04:53:43.950.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kathirvel Alagesan
SpeciesList	scientific name: Pseudomonas entomophila L48; NCBI TaxID: NEWT:384676;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-03 20:38:58	ID requested
⏵ 1	2026-08-18 04:53:44	announced

Dataset with its publication pending

submitter keyword: defecation, Pseudomonas entomophila, pore-forming toxin, Monalysin, proteomics,Intestinal peristalsis, Hfq, Drosophila melanogaster

Kathirvel Alagesan
contact affiliation	Max Planck Unit for the Science of Pathogens Charitéplatz 1 | D-10117 Berlin | Germany
contact email	alagesan@mpusp.mpg.de
lab head
Kathirvel Alagesan
contact affiliation	Max Planck Unit for the Science of Pathogens
contact email	alagesan@mpusp.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD071560
     1. Label: PRIDE project
     2. Name: A Pore-Forming Toxin Monalysin Contributes to Infection-Induced Suppression of Defecation in Female Drosophila