PXD071492-1

PXD071492 is an original dataset announced via ProteomeXchange.

Title	Longitudinal Plasma Nano-proteomics Analysis Reveals Acute Systemic Responses to Radiotherapy and Predictive Biomarkers of Late Toxicity
Description	Radiotherapy induces systemic changes beyond the targeted tumour site, yet the biological mechanisms driving these effects remain poorly understood. Here, we apply the Nano-proteomics pipeline to longitudinally profile systemic responses to radiotherapy in patients with prostate, bladder, and head and neck cancers. Weekly plasma samples collected before and during treatment were analysed by mass spectrometry and revealed acute plasma proteomic changes. The most significant systemic effects occurred within the first two weeks of radiotherapy, highlighting a critical window for biomarker discovery. Across all cohorts, common biological processes were enriched, characterized by early activation of inflammatory and immune pathways, followed by later structural reorganization and immune resolution, regardless of irradiated tissue or concurrent treatments. Despite this shared acute response, distinct protein mediators were dysregulated in a tumour-specific manner. Notably, in prostate cancer patients, baseline proteomics revealed 28 biomarkers associated with late bowel and urinary toxicities, supporting the potential of plasma proteomic biomarkers to identify patients at increased risk of radiotherapy-induced toxicity. This study underscores the value of longitudinal proteomics in uncovering systemic effects of radiotherapy and paves the way for developing predictive biomarkers to guide personalized radiotherapy strategies aimed at minimizing adverse effects.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_21:25:33.959.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD071492
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hanan Abumanhal Masarweh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-02 17:41:40	ID requested
⏵ 1	2026-06-07 21:25:34	announced

Abumanhal-Masarweh H, Assi SA, Liu X, Guerrero Quiles C, Lodhi T, Williams KJ, Cheadle EJ, Kostarelos K, Choudhury A, Wedge DC, West CML, Hadjidemetriou M, Longitudinal plasma nano-proteomics reveals acute systemic responses to radiotherapy and predictive biomarkers of late toxicity. Commun Med (Lond), 6(1):(2026) [pubmed]

10.1038/s43856-026-01552-3;

10.6019/PXD071492;

submitter keyword: Longitudinal,Plasma , Human, Radiotherapy

Marilena Hadjidemetriou
contact affiliation	NanoOmics Lab, Centre for Nanotechnology in Medicine, Division of Cancer Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK.
contact email	marilena.hadjidemetriou@manchester.ac.uk
lab head
Hanan Abumanhal Masarweh
contact affiliation	The University of Manchester
contact email	hanan.abumanhal-masarweh@manchester.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD071492
     1. Label: PRIDE project
     2. Name: Longitudinal Plasma Nano-proteomics Analysis Reveals Acute Systemic Responses to Radiotherapy and Predictive Biomarkers of Late Toxicity