PXD071459-1

PXD071459 is an original dataset announced via ProteomeXchange.

Title	Denervated muscle promotes nerve regeneration via retrograde metabolic signaling, revising the neural-dominated view of neuromuscular interactions
Description	Peripheral nerve injury causes muscle atrophy due to slow axonal regeneration, highlighting an unmet therapeutic need. Although neuromuscular interactions are classically viewed as unidirectionally nerve-dominated, we show that acutely denervated muscle (adMu) regulates nerve regeneration via a retrograde signaling pathway. adMu initiates a trans-tissue regulatory mechanism through extracellular vesicles (EVsadMu) that orchestrate neural energy homeostasis to accelerate regeneration. Functional profiling identifies IDH2 and CS as key metabolic enzymes within EVsadMu. Neurons treated with EVsadMu exhibit a 1.39-fold increase in NADPH/NADP+ ratio via IDH2, along with a 1.18- and 1.27-fold increase in NADH/NAD+ and FADH2/FAD ratios via CS, fueling the TCA cycle to enhance mitochondrial bioenergetics. This restores redox balance and energy supply, driving axonal regeneration. In sciatic nerve injury models, EVadMu-microneedle conduits significantly promote energy metabolism and functional recovery. Together, our findings position adMu as a metabolic signaling center enabling retrograde regulation for nerve regeneration, offering potential for clinical translation.
HostingRepository	iProX
AnnounceDate	2025-12-02
AnnouncementXML	Submission_2025-12-02_00:37:06.222.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qi Liu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-02 00:36:44	ID requested
⏵ 1	2025-12-02 00:37:06	announced

Dataset with its publication pending

submitter keyword: peripheral nerve injury, mitochondrial metabolism, extracellular vesicles, muscle, acute denervation

Bing Xia
contact affiliation	Air Force Medical University
contact email	xiabing8807@fmmu.edu.cn
lab head
Qi Liu
contact affiliation	Air Force Medical University
contact email	1365496218@qq.com
dataset submitter

iProX dataset URI