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PXD071420-1

PXD071420 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMechanistic insights bidirectional extracellular electron transfer driving synchronous nitrogen fixation in Klebsiella variicola
DescriptionMicroorganisms can function as anode catalysts to achieve biological nitrogen fixation while simultaneously generating bioelectricity. To date, only a limited number of microorganisms are capable of performing both outward and inward extracellular electron transfer (EET) concurrently. However, the variations in nitrogen fixation performance and underlying mechanisms during bidirectional EET remain poorly understood. Here, we isolated a nitrogen-fixing bacterium, Klebsiella variicola C1, which exhibits the ability for bidirectional electron transfer. Three-dimensional fluorescence and cyclic voltammetry analyses uncovered distinct differences in biofilm compositions at the anode and cathode. Fluorescence quantitative PCR and comparative proteomics highlighted specific distinctions in key genes and proteins associated with outward and inward EET processes. Outer-membrane lipoprotein carrier proteins likely play a primary role in outward electron transfer, whereas pilus proteins may predominantly facilitate inwa
HostingRepositoryiProX
AnnounceDate2025-12-01
AnnouncementXMLSubmission_2025-12-01_01:18:55.807.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYubiao Lu
SpeciesList scientific name: Klebsiella variicola UCI 18; NCBI TaxID: 1400171;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-12-01 01:18:37ID requested
12025-12-01 01:18:56announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: nitrogen fixation, bidirectional EET, comparative proteomics, Klebsiella variicola
Contact List
Peng Li
contact affiliationZhejiang Ocean University
contact emaillipengwo@zjou.edu.cn
lab head
Yubiao Lu
contact affiliationZhejiang Ocean University
contact email2023111801017@zjou.edu.cn
dataset submitter
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