PXD071392 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The mass spectrometry analysis of CTDP1 function |
| Description | We previously identified an interaction between the RNA polymerase II (Pol II) subunit RPB7 and the phosphatase CTDP1 in regulating transcription termination-reinitiation. Here, we establish that CTDP1 dephosphorylates a unique Pol II phospho-isoform modified at Tyr1, Ser5, and Ser7 of its C-terminal domain. This activity is critical for recruiting the Mediator complex and modulating transcriptional efficiency. Furthermore, by integrating phosphoproteomic and immunoprecipitation-mass spectrometry data, we reveal that CTDP1 also governs the phosphorylation of numerous RNA splicing factors. Our findings position CTDP1 as a master coordinator that integrates transcription with RNA splicing. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-10 |
| AnnouncementXML | Submission_2026-07-10_00:29:51.809.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Haonan Zheng |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | phosphorylated residue |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-30 01:37:32 | ID requested | |
| ⏵ 1 | 2026-07-10 00:29:52 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Pol II, Mediator, CTDP1 |
Contact List
| Xiong Ji |
|---|
| contact affiliation | Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China. |
| contact email | xiongji@pku.edu.cn |
| lab head | |
| Haonan Zheng |
|---|
| contact affiliation | Peking University |
| contact email | zhenghn@pku.edu.cn |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD071392 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071392
- Label: PRIDE project
- Name: The mass spectrometry analysis of CTDP1 function
