PXD071371 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Scube2 primes Dispatched and ADAM10-mediated Shh release by recruiting HDL acceptors to the plasma membrane |
| Description | Sonic hedgehog (Shh) morphogens are lipidated proteins that firmly attach to the outer plasma membrane (PM) of the cells that produce them. The process by which Shh is solubilized includes the transmembrane protein Dispatched1 (Disp), the soluble glycoprotein Scube2, the proteolytic removal of lipidated peptide termini, and the use of soluble lipoproteins (LPPs) as Shh transporters. However, their molecular interplay remains controversial. Here, we demonstrate that A Disintegrin and Metalloproteinase 10, Scube2, and Disp act synergistically to remove Shh from the PM and transfer it to LPP acceptors. We also demonstrate physical Scube2 interactions with LPPs and that these interactions increase Shh release. Finally, we demonstrate that Scube2 strongly binds to heparan sulfate (HS) on cell surfaces. These findings reveal Scube2’s previously unknown role in binding low-abundance, soluble LPP carriers for Shh and recruiting these carriers to HS-rich Shh release sites at the PM to enhance morphogen release. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-23 |
| AnnouncementXML | Submission_2026-02-22_16:12:21.804.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Janna Puschmann |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-28 09:11:00 | ID requested | |
| ⏵ 1 | 2026-02-22 16:12:22 | announced | |
Publication List
| 10.1038/s42003-025-09466-x; |
| Puschmann J, Steffes G, Froese J, Manikowski D, Ehring K, Wittke J, Garbers C, Wegner SV, Grobe K, Scube2 primes Dispatched and ADAM10-mediated Shh release by recruiting HDL acceptors to the plasma membrane. Commun Biol, 9(1):189(2026) [pubmed] |
Keyword List
| submitter keyword: A Disintegrin and Metalloproteinase 10, cholesterol, Heparan sulfate, Scube2, Dispatched, Hedgehog, high-density lipoprotein |
Contact List
| Kay Grobe |
|---|
| contact affiliation | Insitute for Physiologcial Chemistry and Pathobiochemistry University of Muenster Waldeyerstraße 15 48149 Münster Germany |
| contact email | kgrobe@uni-muenster.de |
| lab head | |
| Janna Puschmann |
|---|
| contact affiliation | University of Muenster |
| contact email | janna.puschmann@uni-muenster.de |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD071371 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071371
- Label: PRIDE project
- Name: Scube2 primes Dispatched and ADAM10-mediated Shh release by recruiting HDL acceptors to the plasma membrane
