PXD071369 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification proteins associated with VgrG3 of Chromobacterium violaceum |
| Description | Bacteria in polymicrobial communities engage in complex interactions, including interbacterial antagonism. In Chromobacterium violaceum, the Type VI Secretion System (T6SS) serves as a critical antibacterial weapon, but the full set of effectors delivered by this system remains not yet completely defined. To comprehensively identify T6SS effectors, we performed co-immunoprecipitation of VgrG3, one of the six VgrGs encoded by C. violaceum, followed by LC–MS/MS analysis. Comparative proteomics revealed a diverse array of VgrG3-associated proteins, including all six VgrG homologs, phospholipase effectors from the Tle1 and Tle5 families, PAAR-domain proteins, Tap-1–like adaptors, and the Rhs-family protein RhsF. Functional analyses confirmed that RhsF acts as a T6SS-dependent antibacterial toxin neutralized by its cognate immunity protein, RhsFi (CV_1430), forming a toxin-immunity pair. X-ray crystallography of the RhsF-CT/RhsFi complex at 1.85 Å resolution revealed structural features of ADP-ribosyltransferases in RhsF and demonstrated that RhsFi inhibits toxicity by directly occluding the catalytic site. Mutagenesis assays further confirmed that RhsF toxicity depends on a catalytic triad composed of residues R1403, Y1456, and E1497. These findings expand the known repertoire of T6SS toxins in C. violaceum and place RhsF as a newly identified antibacterial effector in this bacterium. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-24 |
| AnnouncementXML | Submission_2026-08-23_16:23:05.573.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Julia Alves |
| SpeciesList | scientific name: Chromobacterium violaceum ATCC 12472; NCBI TaxID: NEWT:243365; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-28 07:48:25 | ID requested | |
| ⏵ 1 | 2026-08-23 16:23:06 | announced | |
Publication List
| Alves JA, Pankov G, Frey AM, Trost M, Sgro GG, Coulthurst SJ, da Silva Neto JF, Global identification of Chromobacterium violaceum T6SS effectors reveals an Rhs antibacterial toxin featuring FIX and ADP-ribosyltransferase domains. J Biol Chem, 302(7):113216(2026) [pubmed] |
| 10.1016/j.jbc.2026.113216; |
Keyword List
| submitter keyword: Chromobacterium violaceum, Type VI Secretion System, Interbacterial competition, Antibacterial effectors |
Contact List
| Jose Freire da Silva |
|---|
| contact affiliation | Departamento de Biologia Celular e Molecular e Bioagentes Patogenicos |
| contact email | jfsneto@usp.br |
| lab head | |
| Julia Alves |
|---|
| contact affiliation | University of Dundee |
| contact email | jalves001@dundee.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071369
- Label: PRIDE project
- Name: Identification proteins associated with VgrG3 of Chromobacterium violaceum
