PXD071309-1

PXD071309 is an original dataset announced via ProteomeXchange.

Title	Candida Albicans mutant LC-MSMS
Description	Candida albicans is a common resident of humans that colonizes multiple body sites in most healthy individuals (e.g., the gut), but can rapidly proliferate and initiate specific microbial programs to cause infections in immunocompromised hosts. However, the molecular mechanisms underlying its commensal-pathogenic switch have not been fully elucidated. Here, we demonstrate that the mitochondrial protein Mcu1, associated with TCA cycle enzymes, plays a crucial role in sensing N-acetylglucosamine (GlcNAc), a key host intestinal signal, and regulates phenotypic switching in C. albicans. Disruption of Mcu1 or key TCA cycle enzymes impairs the ability of mutant strains to utilize GlcNAc as a carbon source, blocks white-opaque switching in vitro, and reduces intestinal colonization in vivo. We further demonstrated that Mcu1 modulates the mitochondrial redox state, which thereby influences respiratory metabolism, including TCA cycle. Furthermore, Mcu1 regulates white-GUT switching by regulating the expression of Wor1, the master transcriptional regulator of white-opaque switching. Since GUT cells represent a colonization-specific morphology that supports commensalism, our findings indicate that Mcu1 and TCA cycle enzymes cooperatively regulate the commensal-pathogenic switch in C. albicans.
HostingRepository	PRIDE
AnnounceDate	2026-01-20
AnnouncementXML	Submission_2026-01-19_23:08:53.268.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MINGYANG Ma
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: NEWT:5476;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-27 04:14:31	ID requested
⏵ 1	2026-01-19 23:08:53	announced

Dataset with its publication pending

submitter keyword: LC-MSMS, TCA cycle,Candida Albicans

Guanghua Huang
contact affiliation	Shanghai Institute of Infectious Disease and Biosecurity, State Key Laboratory of Genetics and Development of Complex Phenotypes, School of Life Sciences, Department of Laboratory Medicine, Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China College of Pharmaceutical Sciences, Southwest University, Chongqing, China
contact email	huanggh@fudan.edu.cn
lab head
MINGYANG Ma
contact affiliation	School of Life Sciences, Fudan University
contact email	myma24@m.fudan.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD071309

PRIDE project URI

• PRIDE
  1. PXD071309
     1. Label: PRIDE project
     2. Name: Candida Albicans mutant LC-MSMS