PXD071291-1

PXD071291 is an original dataset announced via ProteomeXchange.

Title	Screening of new antibacterial agents against Streptococcus suis and study on their antibacterial mechanism
Description	Background Streptococcus suis infection has attracted much attention in animal medicine and public health, and poses a threat to pig breeding and human health. There is an urgent need to develop highly effective antimicrobial agents. Methods and results The antibacterial efficacy of Ginsenoside Rk1 against Streptococcus suis serotype 2 (SS2) strain ZY05719 was evaluated both in vitro and in vivo. In vitro mechanistic studies revealed that the compound damaged bacterial morphology, inhibited growth and biofilm formation, and induced bacterial DNA leakage. Furthermore, Ginsenoside Rk1 significantly impaired bacterial adherence, survival in whole blood, and resistance to phagocytosis, without exhibiting cytotoxicity to host cells at the tested concentrations. In a mouse model of SS2 infection, Ginsenoside Rk1 treatment significantly enhanced survival rates and alleviated clinical symptoms. Subsequent analysis showed a marked reduction in bacterial loads in the blood, brain, lung, and spleen, alongside decreased expression levels of key inflammatory cytokines (TNF-α, IL-6, IL-1β, CXCL1, and CXCL10) in the treated group. Conclusion The results of this study demonstrate that the novel compound exhibits potent bactericidal activity against Streptococcus suis. It significantly inhibits bacterial growth and biofilm formation, and induces DNA leakage. Furthermore, the compound reduces the bacterial invasiveness to host cells without showing cytotoxic effects. In a mouse model infected with SS2, ginsenoside Rk1 improved survival rates, alleviated clinical symptoms, and reduced bacterial loads in the blood, brain, lungs, and spleen. These multi-target mechanisms support ginsenoside Rk1 as a promising candidate for the treatment of SS2 infections.
HostingRepository	iProX
AnnounceDate	2025-11-27
AnnouncementXML	Submission_2025-11-26_18:56:55.274.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jinwang Hu
SpeciesList	scientific name: Sus scrofa domesticus; NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-26 18:56:41	ID requested
⏵ 1	2025-11-26 18:56:55	announced

Dataset with its publication pending

submitter keyword: Streptococcus suis, cell membrane permeability, inflammatory factors, virulence factors

Yue Zhang
contact affiliation	Animal Molecular Pathogen Laboratory of the Animal Medical College of Henan Agricultural University
contact email	yueruda@163.com
lab head
Jinwang Hu
contact affiliation	Animal Molecular Pathogen Laboratory of the Animal Medical College of Henan Agricultural University
contact email	m17329270701@163.com
dataset submitter

iProX dataset URI