PXD071276 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | NT5C1B improves the fertility of boar spermatozoa by enhanc-ing the quality and cryotolerance during cryopreservation |
| Description | Based on a systematic evaluation of post-thaw characteristics, this study categorizes boar sperm into two distinct cryo-survival phenotypes: cryo-tolerant and cryo-sensitive. The freeze-tolerant group exhibited significantly superior post-thaw quality, including better motility, plasma membrane integrity, acrosome integrity, DNA integrity, and mitochondrial activity. Furthermore, these sperm demonstrated stronger antioxidant capacity and reduced oxidative damage (lower MDA levels). Through proteomic analysis, we identified seven key proteins associated with cold tolerance (e.g., HSPA12A, PRDX4). Functional validation revealed that supplementing the freezing extender with 1 µg/ml recombinant NT5C1B protein significantly improved multiple quality parameters in freeze-sensitive sperm, confirming its role as an effective factor in enhancing cryo-survival. In contrast, ADA protein supplementation showed no significant improvement. The well-established freezing protocol developed in this study provides additional options for boar semen cryopreservation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-05 |
| AnnouncementXML | Submission_2026-01-04_16:21:05.447.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | 士斌 王 |
| SpeciesList | scientific name: Sus scrofa (Pig); NCBI TaxID: NEWT:9823; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-26 12:43:24 | ID requested | |
| ⏵ 1 | 2026-01-04 16:21:06 | announced | |
Publication List
| 10.3390/ani15243530; |
| Wang S, Shi L, Zhang Z, Liu J, Xing J, Yang J, Duan J, Li B, Cao G, NT5C1B Improves Fertility of Boar Spermatozoa by Enhancing Quality and Cryotolerance During Cryopreservation. Animals (Basel), 15(24):(2025) [pubmed] |
Keyword List
| submitter keyword: NT5C1B |
| boar sperm |
| fertility |
| cryopreservation |
| cryotolerance |
Contact List
| Shibin Wang |
|---|
| contact affiliation | Mingxian South Road, Taigu District, Jinzhong City, Shanxi Province, China |
| contact email | wang00bin@163.com |
| lab head | |
| 士斌 王 |
|---|
| contact affiliation | Shanxi Agricultural University |
| contact email | wang0907bin@163.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD071276 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071276
- Label: PRIDE project
- Name: NT5C1B improves the fertility of boar spermatozoa by enhanc-ing the quality and cryotolerance during cryopreservation
